CRISP-ID: decoding CRISPR mediated indels by Sanger sequencing

被引：150

作者：

Dehairs, Jonas ^{[1
]}

Talebi, Ali ^{[1
]}

Cherifi, Yacine ^{[2
]}

Swinnen, Johannes V. ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Dept Oncol, Lab Lipid Metab & Canc, B-3000 Leuven, Belgium

[2] genOway, F-69007 Lyon, France

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

D O I：

10.1038/srep28973

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The advent of next generation gene editing technologies has revolutionized the fields of genome engineering in allowing the generation of gene knockout models and functional gene analysis. However, the screening of resultant clones remains challenging due to the simultaneous presence of different indels. Here, we present CRISP-ID, a web application which uses a unique algorithm for genotyping up to three alleles from a single Sanger sequencing trace, providing a robust and readily accessible platform to directly identify indels and significantly speed up the characterization of clones.

引用

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