Sanguisorba officinalis L. extracts activate Wnt/β-catenin pathway, and subsequently control adipo-osteogenic differentiation

被引：17

作者：

Ji, Hyeongjoon ^{[1
]}

Ahn, Kyuhyun ^{[1
]}

Cho, Hyejoung ^{[1
]}

Kim, Hye-Eun ^{[1
]}

Kim, Young ^{[1
]}

Kim, Okjoon ^{[1
]}

机构：

[1] Chonnam Natl Univ, Dent Sci Res Inst, Sch Dent, Dept Oral Pathol, 33 Yongbong Ro, Gwangju 61186, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 504卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

Sangusorba officinalis L; Wnt/beta-catenin pathway; Adipogenesis; Osteogenesis; OSTEOPOROSIS; WNT; OBESITY; STEM; ADIPOGENESIS; PREVENTION; DISEASE; MARROW; CELLS;

D O I：

10.1016/j.bbrc.2018.08.196

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Wnt/beta-catenin pathway plays an important role in adipogenesis and osteogenesis. To search for novel activators of the Wnt/beta-catenin pathway, we screened plant extracts and found that Sangusorba officinalis L extracts (SOE) could increase beta-catenin expression level and nuclear accumulation in 3T3-L1 and MC3T3-E1 cells. It was confirmed that SOE could effectively control adipo-osteogenic differentiation. SOE inhibited adipocyte differentiation of 3T3-L1 cells, markedly decreased intracellular lipid accumulation, and decreased expression levels of key adipogenic transcription factors including PPAR gamma, C/E13P alpha, and SREBP-1c. SOE enhanced ALP activity and terminal osteoblast differentiation which was confirmed by Alizarin Red S staining of MC3T3-E1 cells. In ex vivo culture, SOE significantly increased the thickness of calvarial bone in mice. Taken together, our results indicate that SOE could be used as a potential therapeutic agent for dual-treatment of anti-obesity and anti-osteoporosis via activation of Wnt/beta-catenin pathway. (C) 2018 Elsevier Inc. All rights reserved.

引用

页码：352 / 358

页数：7