E-P-METHOXYCINNAMIC ACID PRODUCTION IN HAIRY ROOT CULTURES OF SCROPHULARIA BUERGERIANA MIQUEL

被引：1

作者：

Park, Sang Un ^{[1
]}

Li, Xiaohua ^{[1
]}

Eom, Seok Hyun ^{[2
]}

Lee, Chung Yeol ^{[3
]}

Lee, Sook Young ^{[4
]}

机构：

[1] Chungnam Natl Univ, Dept Crop Sci, Taejon 305764, South Korea

[2] Kyung Hee Univ, Dept Hort Biotechnol, Yongin 446701, Gyeonggido, South Korea

[3] Pusan Natl Univ, Dept Plant Biosci, Miryang 627706, Gyeongsangnam, South Korea

[4] Chosun Univ, Res Ctr Oral Dis Regulat Aged, Kwangju 501759, South Korea

来源：

ARCHIVES OF BIOLOGICAL SCIENCES | 2010年 / 62卷 / 03期

关键词：

Agrobacterium rhizogenes; E-p-methoxycinnamic acid (MCA); hairy root; Scrophularia buergeriana Miquel; GROWTH;

D O I：

10.2298/ABS1003649P

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

E-p-methoxycinnamic acid (MCA) is one of the main active constituents of Scrophularia buergeriana Miguel and has hepatoprotective, anti-amnestic, and neuroprotective activities. For studying in vitro production of MCA, we established a hairy root culture of S. buergeriana by infecting leaf explants with Agrobacterium rhizogenes R1000, and tested the growth and MCA production of these cultures using different strengths of media and concentrations of auxins. Hairy roots grown in half-strength MS medium showed the highest levels of growth (10.3 g/l) as well as MCA production (0.83 mg/ 100g dry weight). Hairy root culture with the treatment of 0.5mg/l IBA produced the highest amount of dry weight (11.8 g/l) and MCA (1.26 mg/100g dry weight) production. These results demonstrate that the hairy root culture of S. buergeriana is a valuable alternative approach for the production of MCA.

引用

页码：649 / 652

页数：4

共 20 条

[1] The effect of plant growth regulators on biomass formation and lobeline production of Lobelia inflata L. hairy root cultures [J].