Activation of mitogen-activated protein kinase pathways induces antioxidant response element-mediated gene expression via a Nrf2-dependent mechanism

被引:310
|
作者
Yu, R
Chen, C
Mo, YY
Hebbar, V
Owuor, ED
Tann, TH
Kong, ANT
机构
[1] Univ Illinois, Coll Pharm, Dept Pharmaceut & Pharmacodynam, Chicago, IL 60612 USA
[2] Univ Illinois, Coll Pharm, Ctr Pharmaceut Biotechnol, Chicago, IL 60612 USA
[3] Baylor Coll Med, Dept Immunol, Houston, TX 77030 USA
关键词
D O I
10.1074/jbc.M004037200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Antioxidant response element (ARE) regulates the induction of a number of cellular antioxidant and detoxifying enzymes. However, the signaling pathways that lead to ARE activation remain unknown. Here, we report that the expression of mitogen-activated protein (MAP) kinase/extracellular signal-regulated kinase kinase kinase 1 (MEKK1), transforming growth factor-beta -activated kinase (TAK1), and apoptosis signal-regulating kinase (ASK1) in HepG2 cells activated the ARE reporter gene, whereas the expression of their dominant-negative mutants impaired ARE activation by the chemicals sodium arsenite and mercury chloride. Coexpression of downstream kinases, MAP kinase kinase 4, MAP kinase kinase 6, and c-Jun NH(2)-terminal kinase-1, but not MAP kinase kinase 3 and p38, augmented ARE activation by MEKK1, TAK1, and ASK1. The coexpression of a basic leucine zipper transcription factor Nrf2 but not c-Jun also greatly enhanced the activation of reporter gene by MEKK1, TAK1, and ASK1; however, a dominant-negative mutant of Nrf2 (NF-ES-related factor 2) blocked this event. Furthermore, when overexpressed, MEKK1, TAK1, and ASK1 induced the expression of heme oxygenase-l, a gene regulated by ARE, and the cotransfection with the dominant-negative mutant of Nrf2 abolished the induction. Taken together, these results suggest that MAP kinase pathways that are activated by MEKK1, TAK1, and ASK1 may link chemical signals to Nrf2, leading to the activation of ARE-dependent genes.
引用
收藏
页码:39907 / 39913
页数:7
相关论文
共 50 条
  • [1] Activation of mitogen-activated protein kinases by green tea polyphenols: Potential signaling pathways in the regulation of antioxidant-responsive element-mediated Phase II enzyme gene expression
    Yu, R
    Jiao, JJ
    Duh, JL
    Gudehithlu, K
    Tan, TH
    Kong, ANT
    CARCINOGENESIS, 1997, 18 (02) : 451 - 456
  • [2] The mitogen-activated protein kinase and JAK-STAT signaling pathways are required for an oncostatin M-responsive element-mediated activation of matrix metalloproteinase 1 gene expression
    Korzus, E
    Nagase, H
    Rydell, R
    Travis, J
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (02) : 1188 - 1196
  • [3] Estrogen activation of cyclic adenosine 5′-monophosphate response element-mediated transcription requires the extracellularly regulated kinase/mitogen-activated protein kinase pathway
    Wade, CNB
    Dorsa, DM
    ENDOCRINOLOGY, 2003, 144 (03) : 832 - 838
  • [4] Rapamycin Induces Mitogen-activated Protein (MAP) Kinase Phosphatase-1 (MKP-1) Expression through Activation of Protein Kinase B and Mitogen-activated Protein Kinase Kinase Pathways
    Rastogi, Ruchi
    Jiang, Zhongliang
    Ahmad, Nisar
    Rosati, Rita
    Liu, Yusen
    Beuret, Laurent
    Monks, Robert
    Charron, Jean
    Birnbaum, Morris J.
    Samavati, Lobelia
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2013, 288 (47) : 33966 - 33977
  • [5] Role of Nrf1 in antioxidant response element-mediated gene expression and beyond
    Biswas, Madhurima
    Chan, Jefferson Y.
    TOXICOLOGY AND APPLIED PHARMACOLOGY, 2010, 244 (01) : 16 - 20
  • [6] Adenovirus infection induces microglial activation: involvement of mitogen-activated protein kinase pathways
    Bhat, NR
    Fan, F
    JOURNAL OF NEUROCHEMISTRY, 2002, 81 : 53 - 53
  • [7] Adenovirus infection induces microglial activation: involvement of mitogen-activated protein kinase pathways
    Bhat, NR
    Fan, F
    BRAIN RESEARCH, 2002, 948 (1-2) : 93 - 101
  • [8] Stretch induces mitogen-activated protein kinase activation and myogenic tone through 2 distinct pathways
    Loufrani, L
    Lehoux, S
    Tedgui, A
    Lévy, BI
    Henrion, D
    ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1999, 19 (12) : 2878 - 2883
  • [9] Calcitonin induces mitogen-activated protein kinase activation through multiple signaling pathways.
    Chen, Y
    Shyu, JF
    Inoue, D
    David, JP
    Horne, WC
    Baron, R
    JOURNAL OF BONE AND MINERAL RESEARCH, 1997, 12 : 58 - 58
  • [10] Mitogen-activated protein kinase activation induces corticotrophin-releasing hormone gene expression in human placenta
    Cheng, YH
    Handwerger, S
    LIFE SCIENCES, 2005, 77 (11) : 1263 - 1272