In Vivo Emergence of Aspergillus terreus with Reduced Azole Susceptibility and a Cyp51a M217I Alteration

被引：54

作者：

Arendrup, Maiken C. ^{[1
]}

Jensen, Rasmus H.

Grif, Katharina ^{[4
]}

Skov, Marianne ^{[2
]}

Pressler, Tacjana ^{[2
]}

Johansen, Helle K. ^{[3
]}

Lass-Floerl, Cornelia ^{[4
]}

机构：

[1] Statens Serum Inst, Unit Mycol, Unit Mycol & Parasitol 43 117, Dept Microbiol Surveillance & Res, DK-2300 Copenhagen S, Denmark

[2] Copenhagen CF Ctr, Dept Paediat, Copenhagen, Denmark

[3] Univ Copenhagen Hosp, Rigshosp, Dept Clin Microbiol, DK-2100 Copenhagen, Denmark

[4] Innsbruck Med Univ, Dept Hyg & Med Microbiol, Innsbruck, Austria

来源：

JOURNAL OF INFECTIOUS DISEASES | 2012年 / 206卷 / 06期

关键词：

MULTIPLE RESISTANCE MECHANISMS; FUMIGATUS; ANTIFUNGAL; ITRACONAZOLE;

D O I：

10.1093/infdis/jis442

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Azole resistance in Aspergillus terreus isolates was explored. Twenty related (MB) and 6 unrelated A. terreus isolates were included. CYP51A sequencing and RAPD genotyping was performed. Five MB isolates were itraconazole susceptible, whereas the minimum inhibitory concentrations (MICs) for 15 MB isolates were elevated (1-2 mg/L). Voriconazole and posaconazole MICs were 0.5-4 and 0.06-0.5 mg/L, respectively, for MB isolates but 0.25-0.5 and <0.03-0.06 mg/L, respectively, for controls. Sequencing identified a Cyp51Ap M217I alteration in all 15 isolates with elevated itraconazole MICs. Genotyping showed that 18 of 20 MB isolates were identical and unique, suggesting endogenous origin. In conclusion, itraconazole resistance in A. terreus was linked to an M217I Cyp51A alteration.

引用

页码：981 / 985

页数：5

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