OPTIMIZATION OF SAPONIN EXTRACTION CONDITIONS FROM Ampelozizyphus amazonicus BY USING EXPERIMENTAL DESIGN AND SURFACE RESPONSE METHODOLOGY

被引：2
|
作者：
dos Santos, Fabiane M. [1 ]
Simoes, Jersika C. [1 ]
Silva, Jefferson R. A. [1 ]
Barthus, Rosangela C. [1 ,2 ]
Poppi, Ronei J. [3 ]
Amaral, Ana C. F. [4 ]
机构：
[1] Univ Fed Amazonas, Dept Quim, Inst Ciencias Exatas, BR-69077000 Manaus, Amazonas, Brazil
[2] Univ Fed Espirito Santo, Dept Quim, Ctr Ciencias Exatas, BR-29060900 Vitoria, ES, Brazil
[3] Univ Estadual Campinas, Dept Quim Analit, Inst Quim, BR-13083862 Campinas, SP, Brazil
[4] Fundacao Oswaldo Cruz, Inst Tecnol Farmacos, BR-21041250 Rio De Janeiro, Brazil
来源：
QUIMICA NOVA | 2011年 / 34卷 / 09期
关键词：
saponin; Ampelozizyphus amazonicus; surface response methodology; BIOLOGICAL-ACTIVITIES; ASSISTED EXTRACTION; ROOTS; DUCKE;
D O I：
10.1590/S0100-40422011000900023
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
OPTIMIZATION OF SAPONIN EXTRACTION CONDITIONS FROM Ampelozizyphus amazonicus BY USING EXPERIMENTAL DESIGN AND SURFACE RESPONSE METHODOLOGY. This works describes the use of experimental design and surface response methodology for optimization of saponin extraction from Ampelozizyphus amazonicus. For this purpose, a method employing extraction based on maceration assisted by ultrasound technique was utilized. The following factors were studied: extraction length of time and solvent composition. The total saponin was determined by using a gravimetric method and the results expressed by their relative proportion to total crude extract. For the specific condition, 60% hydro-alcoholic solution and 18 minutes extraction length of time has shown the best results. This method can be useful for extraction of substances with biological importance.
引用
收藏
页码：1629 / 1633
页数：5
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