Detection and Characterization of VIM-31, a New Variant of VIM-2 with Tyr224His and His252Arg Mutations, in a Clinical Isolate of Enterobacter cloacae

被引：13

作者：

Bogaerts, Pierre ^{[1
]}

Bebrone, Carine ^{[2
]}

Huang, Te-Din ^{[1
]}

Bouchahrouf, Warda ^{[1
]}

DeGheldre, Yves ^{[3
]}

Deplano, Ariane ^{[4
]}

Hoffmann, Kurt ^{[2
]}

Glupczynski, Youri ^{[1
]}

机构：

[1] Catholic Univ Louvain, CHU Mt Godinne, Bacteriol Lab, Yvoir, Belgium

[2] Rhein Westfal TH Aachen, Inst Mol Biotechnol, Aachen, Germany

[3] CHIREC Edith Cavell Hosp, Bacteriol Lab, Brussels, Belgium

[4] Erasme ULB Univ Hosp, Bacteriol Lab, Brussels, Belgium

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2012年 / 56卷 / 06期

关键词：

METALLO-BETA-LACTAMASE; PSEUDOMONAS-AERUGINOSA; KLEBSIELLA-PNEUMONIAE; BIOCHEMICAL-CHARACTERIZATION; DISSEMINATION; RESISTANCE; EMERGENCE; DNA; IDENTIFICATION; INTEGRONS;

D O I：

10.1128/AAC.06249-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report the first description of the metallo-beta-lactamase VIM-31, a new variant of VIM-2 with Tyr224His and His252Arg mutations, in Enterobacter cloacae 11236, which was isolated from blood specimens of a patient with colonic adenocarcinoma in Belgium. bla(VIM-31), was found on a class 1 integron located on a self-transferable but not typeable 42-kb plasmid. Compared to values published elsewhere for VIM-2, the purified VIM-31 enzyme showed weaker catalytic efficiency against all the tested beta-lactam agents (except for ertapenem), resulting from lower k(cat) (except for ertapenem) and higher K-m values for VIM-31.

引用

页码：3283 / 3287

页数：5

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