Surface Labeling of Enveloped Viruses Assisted by Host Cells

被引：39

作者：

Huang, Bi-Hai ^{[1
,2
]}

Lin, Yi ^{[1
,2
]}

Zhang, Zhi-Ling ^{[1
,2
]}

Zhuan, Fangfang ^{[3
]}

Liu, An-An ^{[1
,2
]}

Xie, Min ^{[1
,2
]}

Tian, Zhi-Quan ^{[1
,2
]}

Zhang, Zhenfeng ^{[3
]}

Wang, Hanzhong ^{[3
]}

Pang, Dai-Wen ^{[1
,2
]}

机构：

[1] Wuhan Univ, Coll Chem & Mol Sci, Key Lab Analyt Chem Biol & Med,Minist Educ, Res Ctr Nanobiol & Nanomed,State Key Lab Virol,MO, Wuhan 430072, Peoples R China

[2] Wuhan Univ, Wuhan Inst Biotechnol, Wuhan 430072, Peoples R China

[3] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China

来源：

ACS CHEMICAL BIOLOGY | 2012年 / 7卷 / 04期

基金：

中国国家自然科学基金;

关键词：

QUANTUM DOTS; PSEUDORABIES VIRUS; SINGLE VIRUS; PROTEINS; TRACKING; VECTORS; EGRESS; SYSTEM;

D O I：

10.1021/cb2001878

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Labeling of virus opens new pathways for the understanding of viruses themselves and facilitates the utilization of viruses in modern biology, medicine, and materials. Based on the characteristic that viruses hijack their host cellular machineries to survive and reproduce themselves, a host-cell-assisted strategy is proposed to label enveloped viruses. By simply feeding Vero cells with commercial 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (sodium salt) (Biotin-Cap-PE), we obtained biotinylated Vero cells whose membrane systems were modified with biotin. Subsequently, pseudorabies viruses (PrV) were cultivated in the biotinylated Vero cells, and the PrV progenies were spontaneously labeled with Biotin-Cap-PE during viral natural assembly process. Since the viral natural assembly process was employed for the labeling, potential threats of genetic engineering and difficulties in keeping viral natural bioactivity were avoided. Importantly this labeling strategy for enveloped virus greatly reduced the technical complexity and allows researchers from different backgrounds to apply it for their specified demands.

引用

页码：683 / 688

页数：6

共 32 条

[1] Reprogrammed viruses as cancer therapeutics: targeted, armed and shielded [J].