Identification and analysis of B cell epitopes of hemagglutinin of H1N1 influenza virus

被引：1

作者：

Feng, Qing ^{[1
,2
,3
]}

Huang, Xiao-Yan ^{[2
]}

Feng, Yang-Meng ^{[1
,2
,3
]}

Sun, Li-jun ^{[1
,2
,3
]}

Sun, Jing-Ying ^{[1
,2
,3
]}

Li, Yan ^{[1
,2
,3
]}

Xie, Xin ^{[4
]}

Hu, Jun ^{[1
,2
,3
]}

Guo, Chun-Yan ^{[1
,2
,3
]}

机构：

[1] Shaanxi Prov Peoples Hosp, Cent Lab, Xian 710068, Shaanxi, Peoples R China

[2] Shaanxi Prov Key Lab Infect & Immune Dis, Xian, Shaanxi, Peoples R China

[3] Res Ctr Cell Immunol Engn & Technol Shaanxi Prov, Xian, Shaanxi, Peoples R China

[4] Northwest Univ, Coll Life Sci, Key Lab Resource Biol & Biotechnol Western China, Minist Educ, Xian, Peoples R China

来源：

ARCHIVES OF MICROBIOLOGY | 2022年 / 204卷 / 09期

关键词：

Monoclonal antibody (mAb); ELISA blocking test; Computer prediction;

D O I：

10.1007/s00203-022-03133-z

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The frequent variation of influenza virus hemagglutinin (HA) antigen is the main cause of influenza pandemic. Therefore, the study of B cell epitopes of HA is of great significance in the prevention and control of influenza virus. In this study, the split vaccine of 2009 H1N1 influenza virus was used as immunogen, and the monoclonal antibodies (mAbs) were prepared by conventional hybridoma fusion and screening techniques. The characteristics of mAbs were identified by ELISA method, Western-blot test and hemagglutination inhibition test (HI). Using the obtained mAbs as a tool, the B cell epitopes of HA were predicted by ELISA blocking test, sandwich ELISA method and computer simulation method. Finally, four mAbs against HA antigen of H1N1 influenza virus were obtained. The results of ELISA and computer prediction showed that there were at least two types of epitopes on HA of influenza virus. The results of this study complemented the existing methods for predicting HA epitopes, and also provided a new method for predicting other pathogenic microorganisms.

引用

页数：11

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