How to screen non-viral gene delivery systems in vitro?

被引：95

作者：

van Gaal, Ethlinn V. B. ^{[1
]}

van Eijk, Roel ^{[1
]}

Oosting, Ronald S. ^{[2
]}

Kok, Robbert Jan ^{[1
]}

Hennink, Wim E. ^{[1
]}

Crommelin, Daan J. A. ^{[1
,3
]}

Mastrobattista, Enrico ^{[1
]}

机构：

[1] Univ Utrecht, UIPS, Dept Pharmaceut, NL-3584 CG Utrecht, Netherlands

[2] Univ Utrecht, UIPS, Dept Psychopharmacol, NL-3584 CG Utrecht, Netherlands

[3] Dutch Top Inst Pharma, Leiden, Netherlands

来源：

JOURNAL OF CONTROLLED RELEASE | 2011年 / 154卷 / 03期

关键词：

Gene delivery; Transfection; DNA; Particle size; Nanoparticles; pEI; DNA/TRANSFERRIN-PEI COMPLEXES; NUCLEAR-TARGETING SEQUENCE; PLASMID DNA; TRANSFECTION EFFICIENCY; LINEAR POLYETHYLENIMINE; SMOOTH-MUSCLE; INTRACELLULAR TRAFFICKING; REPORTER GENE; DEPENDENT INTERNALIZATION; MEDIATED TRANSFECTION;

D O I：

10.1016/j.jconrel.2011.05.001

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Screening of new gene delivery candidates regarding transfection efficiency and toxicity is usually performed by reading out transgene expression levels relative to a reference formulation after in vitro transfection. However, over the years and among different laboratories, this screening has been performed in a variety of cell lines, using a variety of conditions and read-out systems, and by comparison to a variety of reference formulations. This makes a direct comparison of results difficult, if not impossible. Reaching a consensus would enable placing new results into context of previous findings and estimate the overall contribution to the improvement of non-viral gene delivery. In this paper we illustrate the sensitivity of transfection outcomes on testing conditions chosen, and propose a screening protocol with the aim of standardization within the field. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：218 / 232

页数：15

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