How to screen non-viral gene delivery systems in vitro?

被引：95

作者：

van Gaal, Ethlinn V. B. ^{[1
]}

van Eijk, Roel ^{[1
]}

Oosting, Ronald S. ^{[2
]}

Kok, Robbert Jan ^{[1
]}

Hennink, Wim E. ^{[1
]}

Crommelin, Daan J. A. ^{[1
,3
]}

Mastrobattista, Enrico ^{[1
]}

机构：

[1] Univ Utrecht, UIPS, Dept Pharmaceut, NL-3584 CG Utrecht, Netherlands

[2] Univ Utrecht, UIPS, Dept Psychopharmacol, NL-3584 CG Utrecht, Netherlands

[3] Dutch Top Inst Pharma, Leiden, Netherlands

来源：

JOURNAL OF CONTROLLED RELEASE | 2011年 / 154卷 / 03期

关键词：

Gene delivery; Transfection; DNA; Particle size; Nanoparticles; pEI; DNA/TRANSFERRIN-PEI COMPLEXES; NUCLEAR-TARGETING SEQUENCE; PLASMID DNA; TRANSFECTION EFFICIENCY; LINEAR POLYETHYLENIMINE; SMOOTH-MUSCLE; INTRACELLULAR TRAFFICKING; REPORTER GENE; DEPENDENT INTERNALIZATION; MEDIATED TRANSFECTION;

D O I：

10.1016/j.jconrel.2011.05.001

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Screening of new gene delivery candidates regarding transfection efficiency and toxicity is usually performed by reading out transgene expression levels relative to a reference formulation after in vitro transfection. However, over the years and among different laboratories, this screening has been performed in a variety of cell lines, using a variety of conditions and read-out systems, and by comparison to a variety of reference formulations. This makes a direct comparison of results difficult, if not impossible. Reaching a consensus would enable placing new results into context of previous findings and estimate the overall contribution to the improvement of non-viral gene delivery. In this paper we illustrate the sensitivity of transfection outcomes on testing conditions chosen, and propose a screening protocol with the aim of standardization within the field. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：218 / 232

页数：15

共 183 条

[1]

Akinc A, 2002, BIOTECHNOL BIOENG, V78, P503, DOI 10.1002/bit.20215

[2] A polymer library approach to suicide gene therapy for cancer [J].

Anderson, DG ;

Peng, WD ;

Akinc, A ;

Hossain, N ;

Kohn, A ;

Padera, R ;

Langer, R ;

Sawicki, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (45) :16028-16033

[3] An improved β-galactosidase reporter gene [J].

Anson, DS ;

Limberis, M .

JOURNAL OF BIOTECHNOLOGY, 2004, 108 (01) :17-30

[4] Delivery of therapeutic shRNA and siRNA by Tat fusion peptide targeting bcr-abl fusion gene in Chronic Myeloid Leukemia cells [J].

Arthanari, Yamini ;

Pluen, Alain ;

Rajendran, Ramkumar ;

Aojula, Harmesh ;

Demonacos, Constantinos .

JOURNAL OF CONTROLLED RELEASE, 2010, 145 (03) :272-280

[5]

*ATCC, 2007, TECHN B ATCC, V7

[6] GENE-TRANSFER OPTIMIZATION WITH LIPOSPERMINE-COATED DNA [J].

BARTHEL, F ;

REMY, JS ;

LOEFFLER, JP ;

BEHR, JP .

DNA AND CELL BIOLOGY, 1993, 12 (06) :553-560

[7] Basic studies on gene therapy of human malignant melanoma by use of the human interferon β gene entrapped in cationic multilamellar liposomes.: 1.: Morphology and growth rate of six melanoma cell lines used in transfection experiments with the human interferon β gene [J].

Benga, G .

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2001, 5 (04) :402-408

[8] Gene transfer in vitro and in vivo by cationic lipids is not significantly affected by levels of supercoiling of a reporter plasmid [J].

Bergan, D ;

Galbraith, T ;

Sloane, DL .

PHARMACEUTICAL RESEARCH, 2000, 17 (08) :967-973

[9] Intracellular route and transcriptional competence of polyethylenimine-DNA complexes [J].

Bieber, T ;

Meissner, W ;

Kostin, S ;

Niemann, A ;

Elsasser, HP .

JOURNAL OF CONTROLLED RELEASE, 2002, 82 (2-3) :441-454

[10] Purification of polyethylenimine polyplexes highlights the role of free polycations in gene transfer [J].

Boeckle, S ;

von Gersdorff, K ;

van der Piepen, S ;

Culmsee, C ;

Wagner, E ;

Ogris, M .

JOURNAL OF GENE MEDICINE, 2004, 6 (10) :1102-1111

← 1 2 3 4 5 6 7 8 9 10 →