Direct Membrane Binding by Bacterial Actin MreB

被引：176

作者：

Salje, Jeanne ^{[1
]}

van den Ent, Fusinita ^{[1
]}

de Boer, Piet ^{[2
]}

Loewe, Jan ^{[1
]}

机构：

[1] MRC Lab Mol Biol, Cambridge CB2 0QH, England

[2] Case Western Reserve Univ, Dept Mol Biol & Microbiol, Cleveland, OH 44106 USA

来源：

MOLECULAR CELL | 2011年 / 43卷 / 03期

基金：

美国国家卫生研究院;

关键词：

CELL-WALL MORPHOGENESIS; ESCHERICHIA-COLI; BACILLUS-SUBTILIS; SHAPE DETERMINATION; CYTOSKELETON; PROTEINS; COMPLEXES; FILAMENTS; HOMOLOG; GENES;

D O I：

10.1016/j.molcel.2011.07.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacterial actin MreB is one of the key components of the bacterial cytoskeleton. It assembles into short filaments that lie just underneath the membrane and organize the cell wall synthesis machinery. Here we show that MreB from both T. maritima and E. coli binds directly to cell membranes. This function is essential for cell shape determination in E. coli and is proposed to be a general property of many, if not all, MreBs. We demonstrate that membrane binding is mediated by a membrane insertion loop in TmMreB and by an N-terminal amphipathic helix in EcMreB and show that purified TmMreB assembles into double filaments on a membrane surface that can induce curvature. This, the first example of a membrane-binding actin filament, prompts a fundamental rethink of the structure and dynamics of MreB filaments within cells.

引用

页码：478 / 487

页数：10

共 29 条

[1] Conditional lethality, division defects, membrane involution, and endocytosis in mre and mrd shape mutants of Escherichia coli [J].