Effect of the traditional Chinese medicine tongxinluo on endothelial dysfunction rats studied by using urinary metabonomics based on liquid chromatography-mass spectrometry

被引：52

作者：

Dai, Weidong ^{[2
]}

Wei, Cong ^{[1
]}

Kong, Hongwei ^{[2
]}

Jia, Zhenhua ^{[1
]}

Han, Jianke ^{[1
]}

Zhang, Fengxia ^{[2
]}

Wu, Zeming ^{[2
]}

Gu, Yan ^{[2
]}

Chen, Shili ^{[2
]}

Gu, Qun ^{[2
]}

Lu, Xin ^{[2
]}

Wu, Yiling ^{[1
]}

Xu, Guowang ^{[2
]}

机构：

[1] Integrat Tradit & Western Med Res Acad Hebei Prov, Shijiazhuang 050035, Hebei Province, Peoples R China

[2] Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2011年 / 56卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Metabonomics; Urine; Endothelial dysfunction; LC-MS; Tongxinluo; Simvastatin; INDOXYL SULFATE; OXIDATIVE STRESS; UREMIC TOXIN; P-CRESOL; HPLC-MS; METABOLOMICS; STRATEGY; SYSTEMS;

D O I：

10.1016/j.jpba.2011.04.020

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A urinary metabonomic method based on ultra-fast liquid chromatography coupled with ion trap-time of flight mass spectrometry (UFLC/MS-IT-TOF) was employed to study the preventive efficacy and the metabolic changes caused by simavastatin and the traditional Chinese medicine tongxinluo in endothelial dysfunction rats. Principal component analysis (PCA) was applied to study metabolic patterns of endothelial dysfunction rats and healthy control rats. 1-Methyladenosine, indoxyl sulfate, hippuric acid, riboflavin, coproporphyrin, and p-cresol glucuronide were identified as potential biomarkers, indicating that pathways of adenine, tryptophan, phenylalanine, riboflavin, and porphyrin metabolism were disturbed in endothelial dysfunction rats. Applications of simvastatin and tongxinluo to endothelial dysfunction rats improved endothelial function according to the results of histopathology and measurements of endothelin-1 and nitric oxide. Metabonomic studies suggested that tongxinluo prevents endothelial dysfunction by regulating multiple metabolic pathways to their normal state, whereas simvastatin only altered selected metabolic pathways. This research demonstrated that metabonomics is a powerful and promising tool for disease investigation and the efficacy evaluation of complex traditional Chinese medicines. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：86 / 92

页数：7

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