Structural characterization of exopolysaccharides from biofilm of a cariogenic streptococci

被引：24

作者：

Aires, Carolina P. ^{[2
]}

Tenuta, Livia M. ^{[3
]}

Carbonero, Elaine R. ^{[4
]}

Sassaki, Guilherme L. ^{[1
]}

Iacomini, Marcello ^{[1
]}

Cury, Jaime A. ^{[3
]}

机构：

[1] Univ Fed Parana, Dept Biochem & Mol Biol, BR-81531980 Curitiba, Parana, Brazil

[2] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Chem & Phys, BR-14040903 Ribeirao Preto, SP, Brazil

[3] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, BR-13414903 Piracicaba, SP, Brazil

[4] Univ Fed Goias, Dept Chem, BR-75704020 Catalao, Go, Brazil

来源：

CARBOHYDRATE POLYMERS | 2011年 / 84卷 / 04期

关键词：

Streptococcus mutans; Biofilm; Chemical structure; Polysaccharides; alpha-Glucans; IN-SITU; SUCROSE; PLAQUE;

D O I：

10.1016/j.carbpol.2010.12.076

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Soluble (EPS-SOL), as well as insoluble extracellular polysaccharide (EPS-INSOL), extracted from biofilm of Streptococcus mutans, were analyzed by nuclear magnetic resonance spectroscopy, methylation analysis, and a controlled Smith degradation. EPS-SOL was a branched alpha-glucan containing a (1 -> 6)-and (1 -> 3)-linkages. EPS-INSOL was a branched alpha-glucan with similar linkages, but with a (1 -> 3)-linked main-chain partially substituted at O-6 with Glcp-(1 -> 6)-Glcp-side chains. Biofilm EPS had a distinct chemical structure compared with those synthesized by plankton cells or by purified enzymes from S. mutans, which could indicate different mechanisms for its degradation. (C) 2011 Published by Elsevier Ltd.

引用

页码：1215 / 1220

页数：6

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