Distinct Subunit Domains Govern Synaptic Stability and Specificity of the Kainate Receptor

被引：30

作者：

Straub, Christoph ^{[1
,2
]}

Noam, Yoav ^{[1
,2
]}

Nomura, Toshihiro ^{[4
]}

Yamasaki, Miwako ^{[5
]}

Yan, Dan ^{[1
,2
]}

Fernandes, Herman B. ^{[4
]}

Zhang, Ping ^{[3
]}

Howe, James R. ^{[3
]}

Watanabe, Masahiko ^{[5
]}

Contractor, Anis ^{[4
]}

Tomita, Susumu ^{[1
,2
]}

机构：

[1] Yale Univ, Sch Med, Dept Cellular & Mol Physiol, New Haven, CT 06520 USA

[2] Yale Univ, Sch Med, Dept Neurosci, CNNR Program, New Haven, CT 06520 USA

[3] Yale Univ, Sch Med, Dept Pharmacol, New Haven, CT 06520 USA

[4] Northwestern Univ, Feinberg Sch Med, Dept Physiol, Chicago, IL 60611 USA

[5] Hokkaido Univ, Grad Sch Med, Dept Anat, Sapporo, Hokkaido 0608638, Japan

来源：

CELL REPORTS | 2016年 / 16卷 / 02期

关键词：

MOSSY-FIBER SYNAPSES; SELECTIVE GLUTAMATE RECEPTORS; ACID BINDING-SITES; DIFFERENTIAL REGULATION; C1Q-LIKE PROTEINS; AUXILIARY SUBUNIT; NERVOUS-SYSTEM; AMPA RECEPTORS; MOUSE-BRAIN; LOCALIZATION;

D O I：

10.1016/j.celrep.2016.05.093

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Synaptic communication between neurons requires the precise localization of neurotransmitter receptors to the correct synapse type. Kainate-type glutamate receptors restrict synaptic localization that is determined by the afferent presynaptic connection. The mechanisms that govern this input-specific synaptic localization remain unclear. Here, we examine how subunit composition and specific subunit domains contribute to synaptic localization of kainate receptors. The cytoplasmic domain of the GluK2 low-affinity subunit stabilizes kainate receptors at synapses. In contrast, the extracellular domain of the GluK4/5 high-affinity subunit synergistically controls the synaptic specificity of kainate receptors through interaction with C1q-like proteins. Thus, the input-specific synaptic localization of the native kainate receptor complex involves two mechanisms that underlie specificity and stabilization of the receptor at synapses.

引用

页码：531 / 544

页数：14

共 47 条

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