Redox imaging using genetically encoded redox indicators in zebrafish and mice

被引：13

作者：

Breckwoldt, Michael O. ^{[1
,2
]}

Wittmann, Christine ^{[3
]}

Misgeld, Thomas ^{[4
,5
,6
,7
]}

Kerschensteiner, Martin ^{[5
,8
]}

Grabher, Clemens ^{[3
]}

机构：

[1] Heidelberg Univ, Dept Neuroradiol, D-69120 Heidelberg, Germany

[2] German Canc Res Ctr, Clin Cooperat Unit Neuroimmunol & Brain Tumor Imm, D-69120 Heidelberg, Germany

[3] Karlsruhe Inst Technol, ITG, D-76344 Eggenstein Leopoldshafen, Germany

[4] Tech Univ Munich, Inst Neuronal Cell Biol, D-80802 Munich, Germany

[5] Munich Cluster Syst Neurol SyNergy, Munich, Germany

[6] Ctr Integrated Prot Sci, Munich, Germany

[7] German Ctr Neurodegenerat Dis, Munich, Germany

[8] Univ Munich, Inst Clin Neuroimmunol, D-81377 Munich, Germany

来源：

BIOLOGICAL CHEMISTRY | 2015年 / 396卷 / 05期

关键词：

genetically encoded redox indicators; Grx1-roGFP2; HyPer; in vivo microscopy; redox signals; roGFP; YELLOW FLUORESCENT PROTEIN; MITOCHONDRIAL OXIDANT STRESS; IN-VIVO; HYDROGEN-PEROXIDE; CELL-DEATH; NEURONS; CALCIUM; SUPEROXIDE; CPYFP; PROBE;

D O I：

10.1515/hsz-2014-0294

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Redox signals have emerged as important regulators of cellular physiology and pathology. The advent of redox imaging in vertebrate systems now provides the opportunity to dynamically visualize redox signaling during development and disease. In this review, we summarize recent advances in the generation of genetically encoded redox indicators (GERIs), introduce new redox imaging strategies, and highlight key publications in the field of vertebrate redox imaging. We also discuss the limitations and future potential of in vivo redox imaging in zebrafish and mice.

引用

页码：511 / 522

页数：12

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