Protective effect of cirsimaritin against streptozotocin-induced apoptosis in pancreatic beta cells

被引：39

作者：

Lee, Dahae ^{[1
]}

Kim, Ki Hyun ^{[1
]}

Lee, Jaemin ^{[2
]}

Hwang, Gwi Seo ^{[3
]}

Lee, Hye Lim ^{[3
]}

Hahm, Dae-Hyun ^{[4
]}

Huh, Chang Ki ^{[5
]}

Lee, Sang Cheon ^{[5
]}

Lee, Sanghyun ^{[2
]}

Kang, Ki Sung ^{[3
]}

机构：

[1] Sungkyunkwan Univ, Sch Pharm, Suwon, South Korea

[2] Chung Ang Univ, Dept Integrat Plant Sci, Ansong 17546, South Korea

[3] Gachon Univ, Coll Korean Med, Songnam 13120, South Korea

[4] Kyung Hee Univ, Acupuncture & Meridian Sci Res Ctr, Seoul, South Korea

[5] Imsil Res Inst Cheese Sci, Imsil, South Korea

来源：

JOURNAL OF PHARMACY AND PHARMACOLOGY | 2017年 / 69卷 / 07期

关键词：

apoptosis; cirsimaritin; Cirsium japonicum var; maackii; streptozotocin; TYPE-1; DIABETES-MELLITUS; POLY(ADP-RIBOSE) POLYMERASE; OXIDATIVE STRESS; IN-VITRO; FLAVONOIDS; ANTIOXIDANT; MECHANISMS; MICE; RATS; SESQUITERPENES;

D O I：

10.1111/jphp.12719

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

ObjectivesMaintaining glucose homoeostasis is essential for the survival of cells. Despite the various health benefits of Korean thistle (Cirsium japonicum var. maackii), their effects on pancreatic -cell apoptosis in type 1 diabetes mellitus and the underlying mechanisms remain unclear, and experimentally investigated in this study. MethodsThe effects of C. japonicum var. maackii and its active component cirsimaritin against streptozotocin (STZ)-induced cytotoxicity were assessed in INS-1 cells. By Western blotting analysis, protein expressions related to apoptosis were evaluated. The involvement of apoptosis was also confirmed with image-based cytometric assay and caspase activity tests. Key findingsCirsium japonicum var. maackii extract and cirsimaritin in non-toxic concentrations improved cell viability to near normal levels and protected INS-1 cells against STZ-induced damage. In addition, cirsimaritin reduced the intracellular oxidative stress induced by STZ. Cirsimaritin effectively suppressed apoptosis in pancreatic cells by decreasing the activation of caspase-8 and caspase-3, BID and the DNA repair protein poly (ADP-ribose) polymerase (PARP) and increasing anti-apoptotic BCL-2 protein expression. ConclusionsThis study demonstrates the therapeutic potential and action mechanism of cirsimaritin for the prevention and treatment of type 1 diabetes mellitus.

引用

页码：875 / 883

页数：9