Development of single-locus DNA microsatellite markers using 5'anchored ISSR-PCR method for the mangrove horseshoe crab, Carcinoscorpius rotundicauda (Latreille, 1802) in Peninsular Malaysia

被引：7

作者：

Adibah, A. B. ^{[1
]}

Ling, L. Pui ^{[1
]}

Tan, S. G. ^{[1
]}

Faridah, Q. Z. ^{[2
]}

Christianus, A. ^{[3
]}

机构：

[1] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Cell & Mol Biol, Serdang 43400, Selangor, Malaysia

[2] Univ Putra Malaysia, Fac Sci, Dept Biol, Serdang 43400, Selangor, Malaysia

[3] Univ Putra Malaysia, Fac Agr, Dept Aquaculture, Serdang 43400, Selangor, Malaysia

来源：

MOLECULAR BIOLOGY REPORTS | 2012年 / 39卷 / 04期

关键词：

Mangrove horseshoe crab; C; rotundicauda; 5 ' anchored ISSR-PCR; Microsatellite;

D O I：

10.1007/s11033-011-1159-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Horseshoe crabs are said to be declining worldwide. However, there is still no published report on the status of horseshoe crabs in Malaysia. Thus, we report here eight informative microsatellite markers that were developed using the 5'-anchored ISSR-PCR enrichment procedure to diagnose the population genetic structure of the mangrove horseshoe crab, Carcinoscorpius rotundicauda from Peninsular Malaysia. This set of markers was tested on 127 samples and showed polymorphism in this species. Hence they should be useful in future essential population genetic studies of these living fossils in the Southeast Asian region.

引用

页码：3815 / 3820

页数：6

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