Simple characterisation of a deformable mirror inside a high numerical aperture microscope using phase diversity

被引:10
作者
Debarre, D. [1 ,2 ]
Vieille, T.
Beaurepaire, E.
机构
[1] Ecole Polytech ParisTech, CNRS, Lab Opt & Biosci, F-91128 Palaiseau, France
[2] INSERM, F-91128 Palaiseau, France
关键词
adaptive optics; microscopy; phase retrieval; wavefront control; ADAPTIVE OPTICS; ABERRATION CORRECTION; 2-PHOTON MICROSCOPY; CALIBRATION;
D O I
10.1111/j.1365-2818.2011.03518.x
中图分类号
TH742 [显微镜];
学科分类号
摘要
We present a simple and versatile scheme for characterising amplitude and phase modulation by an active element, such as a deformable mirror, in the pupil plane of a high NA microscope. By placing a mirror in the vicinity of the focal plane of the objective and recording images of the reflected focal spot on a camera, we show that reliable measurements of the influence function of the mirror actuators in the pupil plane of the objective can be obtained using an iterative electric field retrieval algorithm. Compared to direct wavefront sensors, the proposed method allows characterisation for a variety of objectives with different NA and pupil sizes without modification of the setup, requires minimal space inside the microscope, and can be used with pulsed sources such as used for multiphoton microscopy. In order to validate our method, we compare our data to the results obtained with a Shack-Hartmann wavefront sensor, and show that comparable precision is achieved.
引用
收藏
页码:136 / 143
页数:8
相关论文
共 13 条
  • [1] Methods for the characterization of deformable membrane mirrors
    Booth, M
    Wilson, T
    Sun, HB
    Ota, T
    Kawata, S
    [J]. APPLIED OPTICS, 2005, 44 (24) : 5131 - 5139
  • [2] Adaptive aberration correction in a confocal microscope
    Booth, MJ
    Neil, MAA
    Juskaitis, R
    Wilson, T
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (09) : 5788 - 5792
  • [3] An optical technique for remote focusing in microscopy
    Botcherby, E. J.
    Juskaitis, R.
    Booth, M. J.
    Wilson, T.
    [J]. OPTICS COMMUNICATIONS, 2008, 281 (04) : 880 - 887
  • [4] Adaptive optics for structured illumination microscopy
    Debarre, Delphine
    Botcherby, Edward J.
    Booth, Martin J.
    Wilson, Tony
    [J]. OPTICS EXPRESS, 2008, 16 (13) : 9290 - 9305
  • [5] Image based adaptive optics through optimisation of low spatial frequencies
    Debarre, Delphine
    Booth, Martin J.
    Wilson, Tony
    [J]. OPTICS EXPRESS, 2007, 15 (13): : 8176 - 8190
  • [6] Image-based adaptive optics for two-photon microscopy
    Debarre, Delphine
    Botcherby, Edward J.
    Watanabe, Tomoko
    Srinivas, Shankar
    Booth, Martin J.
    Wilson, Tony
    [J]. OPTICS LETTERS, 2009, 34 (16) : 2495 - 2497
  • [7] Phase-retrieved pupil functions in wide-field fluorescence microscopy
    Hanser, BM
    Gustafsson, MGL
    Agard, DA
    Sedat, JW
    [J]. JOURNAL OF MICROSCOPY, 2004, 216 (01) : 32 - 48
  • [8] Phase retrieval for high-numerical-aperture optical systems
    Hanser, BM
    Gustafsson, MGL
    Agard, DA
    Sedat, JW
    [J]. OPTICS LETTERS, 2003, 28 (10) : 801 - 803
  • [9] Calibration of a deformable mirror and Strehl ratio measurements by use of phase diversity
    Löfdahl, MG
    Scharmer, GB
    Wei, W
    [J]. APPLIED OPTICS, 2000, 39 (01) : 94 - 103
  • [10] Practical implementation of adaptive optics in multiphoton microscopy
    Marsh, PN
    Burns, D
    Girkin, JM
    [J]. OPTICS EXPRESS, 2003, 11 (10): : 1123 - 1130