Involvement of cellular cytoskeleton components in antibody-induced internalization of viral glycoproteins in pseudorabies virus-infected monocytes

被引:19
作者
Van de Walle, GR
Favoreel, HW
Nauwynck, HJ
Van Oostveldt, P
Pensaert, MB
机构
[1] Univ Ghent, Fac Vet Med, Virol Lab, B-9000 Ghent, Belgium
[2] Univ Ghent, Fac Appl Biol & Agr Sci, Lab Biochem & Mol Cytol, B-9000 Ghent, Belgium
关键词
antibodies; pseudorabies virus; endocytosis; cytoskeleton;
D O I
10.1006/viro.2001.1064
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Addition of pseudorabies virus (PrV)-specific polyclonal immunoglobulins to PrV-infected monocytes induces internalization of plasma membrane-anchored viral glycoproteins. and this may interfere with anti body-dependent cell lysis. We investigated the role of actin, microtubules, clathrin, and dynein, the major cellular components involved in physiological endocytosis during this virological internalization. Porcine monocytes were infected in vitro for 13 h and afterward treated with different concentrations of colchicine, cytochalasin D, latrunculin B, and amantadine-HCl, which inhibit polymerization of microtubules, actin/clathrin, actin, and clathrin, respectively, This resulted in a significant reduction of internalization compared to the nontreated control, indicating that these components are involved in the process. A double labeling was performed during the internalization process and a clear colocalization of actin, microtubules, clathrin, and dynein with the viral glycoproteins was observed at different stages during the internalization process. We conclude that these cellular components are used by PrV to generate the antibody-induced internalization of viral glycoproteins. (C) 2001 Academic Press.
引用
收藏
页码:129 / 138
页数:10
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