Stoichiometry of dipalmitoylphosphatidylcholine-DNA interaction in the presence of Ca2+:: a temperature-scanning ultrasonic study

被引：35
|
作者：
Kharakoz, DP [1 ]
Khusainova, RS
Gorelova, AV
Dawson, KA
机构：
[1] Russian Acad Sci, Inst Theoret & Expt Biophys, Pushchino 142292, Russia
[2] Univ Coll Dublin, Irish Ctr Colloid Sci & Biomat, Dept Chem, Dublin 4, Ireland
来源：
FEBS LETTERS | 1999年 / 446卷 / 01期
关键词：
DNA-lipid interaction; phase transition; transfection; ultrasonics;
D O I：
10.1016/S0014-5793(99)00165-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA-DPPC complexes can be prepared by means of a single step procedure of mixing DNA solution and aqueous lipid dispersion in the presence of calcium ions. Interaction between DPPC and DNA brings about a biphasic shape of melting curves corresponding to the free lipid and the strongly bound one. The amount of the strongly bound lipid is 5 molecules per nucleotide which is close to the size of the first lipid monolayer around DNA molecule. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码：27 / 29
页数：3
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