Differential Multiphoton Laser Scanning Microscopy

被引：10

作者：

Field, Jeffrey J. ^{[1
]}

Sheetz, Kraig E. ^{[2
]}

Chandler, Eric V. ^{[1
]}

Hoover, Erich E. ^{[1
]}

Young, Michael D. ^{[1
]}

Ding, Shi-you ^{[3
]}

Sylvester, Anne W. ^{[4
]}

Kleinfeld, David ^{[5
]}

Squier, Jeff A. ^{[1
]}

机构：

[1] Colorado Sch Mines, Dept Phys, Ctr Microintegrated Opt Adv Bioimaging & Control, Golden, CO 80401 USA

[2] US Mil Acad, Dept Phys & Nucl Engn, West Point, NY 10996 USA

[3] Natl Renewable Energy Lab, Golden, CO 80401 USA

[4] Univ Wyoming, Dept Mol Biol, Laramie, WY 82071 USA

[5] Univ Calif San Diego, Dept Phys, La Jolla, CA 92093 USA

来源：

IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS | 2012年 / 18卷 / 01期

基金：

美国国家科学基金会;

关键词：

Fluorescence microscopy; nonlinear microscopy; nonlinear optics; second-harmonic generation (SHG); two-photon microscopy; ultrafast optics; FLUORESCENCE MICROSCOPY; 2-PHOTON EXCITATION; SUPERCONTINUUM GENERATION; LIGHT-SOURCE; RESOLUTION; COHERENT; DEPTH; FIELD; DIFFRACTION; POLARIZATION;

D O I：

10.1109/JSTQE.2010.2077622

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Multifocal multiphoton laser scanning microscopy (mfMPLSM) in the biological and medical sciences has the potential to become a ubiquitous tool for obtaining high-resolution images at video rates. While current implementations of mfMPLSM achieve very high frame rates, they are limited in their applicability to essentially those biological samples that exhibit little or no scattering. In this paper, we report on a method for mfMPLSM in which whole-field detection with a single detector, rather than detection with a matrix of detectors, such as a charge-coupled device (CCD) camera, is implemented. This advance makes mfMPLSM fully compatible for use in imaging through scattering media. Further, we demonstrate that this method makes it possible to simultaneously obtain multiple images and view differences in excitation parameters in a single scan of the specimen.

引用

页码：14 / 28

页数：15

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