Stability of nitroblue tetrazolium-based alkaline phosphatase substrates

被引：17
作者：
Smejkal, GB
Kaul, CA
机构：
[1] Cleveland Clin Fdn, Dept Clin Pathol, Cleveland, OH 44195 USA
[2] Lerner Res Inst, Dept Cell Biol, Cleveland, OH USA
来源：
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2001年 / 49卷 / 09期
关键词：
alkaline phosphatase; 5-bromo-4-chloro-3-indolyl phosphate; formazan; nitroblue tetrazolium chloride; von Willebrand factor; Western blotting;
D O I：
10.1177/002215540104900914
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
This report demonstrates the stability of NBT substrate after multiple exposures to alkaline phosphatase. Perhaps more important than the ability to reuse substrates, the report provides some insight into the mechanisms by which tetrazoliums are reduced and evidence for the formation of an intermediary product, i.e., a half-formazan that is reduced more rapidly.
引用
收藏
页码：1189 / 1190
页数：2
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