Biosensors for assay of glycoalkaloids in potato tubers

被引：8
|
作者：
Arkhypova, V. N. [1 ]
Dzyadevych, S. V. [1 ]
Jaffrezic-Renault, N. [2 ]
Martelet, C. [2 ]
Soldatkin, A. P. [1 ]
机构：
[1] Natl Acad Sci Ukraine, Inst Mol Biol & Genet, UA-03143 Kiev, Ukraine
[2] Ecole Cent Lyon, F-69134 Ecully, France
来源：
APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2008年 / 44卷 / 03期
关键词：
D O I：
10.1134/S0003683808030162
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The possibility of practical application of biosensors based on pH-sensitive field-effect transistors and butyrylcholinesterase to glycoalkaloid analysis in potato tubers was studied. The main analytical features of the designed biosensors and measurement conditions were optimized. The biosensor was applied to quantitative analysis of glycoalkaloids in tubers of different potato varieties. The results proved to be in good agreement with those obtained by conventional protocols. Experiments on glucose assay were performed. An inverse correlation between the contents of glucose and glycoalkaloids in potato tubers was demonstrated.
引用
收藏
页码：314 / 318
页数：5
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