Diverse pathogenicity of Burkholderia cepacia complex strains in the Caenorhabditis elegans host model

被引：43
作者：
Cardona, ST
Wopperer, J
Eberl, L
Valvano, MA
机构：
[1] Univ Western Ontario, Dept Microbiol, London, ON N6A 5C1, Canada
[2] Univ Western Ontario, Dept Immunol, Infect Dis Res Grp, Siebens Drake Res Inst, London, ON N6A 5C1, Canada
[3] Univ Zurich, Dept Microbiol, Zurich, Switzerland
[4] Univ Western Ontario, Dept Med, Infect Dis Res Grp, Siebens Drake Res Inst, London, ON N6A 5C1, Canada
来源：
FEMS MICROBIOLOGY LETTERS | 2005年 / 250卷 / 01期
基金：
加拿大健康研究院;
关键词：
Burkholderia cepacia complex; Caenorhabditis elegans; host model; pathogenicity screening;
D O I：
10.1016/j.femsle.2005.06.050
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A fast screening method was developed to assess the pathogenicity of a diverse collection of environmental and clinical Burkholderia cepacia complex isolates in the nematode Caenorhabditis elegans. The method was validated by comparison with the standard slow-killing assay. We observed that the pathogenicity of B. cepacia complex isolates in C elegans was strain-dependent but species-independent. The wide range of observed pathogenic phenotypes agrees with the high degree of phenotypic variation among species of the R cepacia complex and suggests that the taxonomic classification of a given strain within the complex cannot predict pathogenicity. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：97 / 104
页数：8
相关论文
共 25 条
[1]   Caenorhabditis elegans as a host for the study of host-pathogen interactions [J].
Aballay, A ;
Ausubel, FM .
CURRENT OPINION IN MICROBIOLOGY, 2002, 5 (01) :97-101
[2]   The Burkholderia cepacia epidemic strain marker is part of a novel genomic island encoding both virulence and metabolism-associated genes in Burkholderia cenocepacia [J].
Baldwin, A ;
Sokol, PA ;
Parkhill, J ;
Mahenthiralingam, E .
INFECTION AND IMMUNITY, 2004, 72 (03) :1537-1547
[3]   Comparative analysis of plant and animal models for characterization of Burkholdetia cepacia virulence [J].
Bernier, SP ;
Silo-Suh, L ;
Woods, DE ;
Ohman, DE ;
Sokol, PA .
INFECTION AND IMMUNITY, 2003, 71 (09) :5306-5313
[4]   Differential persistence among genomovars of the Burkholderia cepacia complex in a murine model of pulmonary infection [J].
Chu, KK ;
Davidson, DJ ;
Halsey, TK ;
Chung, JW ;
Speert, DP .
INFECTION AND IMMUNITY, 2002, 70 (05) :2715-2720
[5]   Diversity and significance of Burkholderia species occupying diverse ecological niches [J].
Coenye, T ;
Vandamme, P .
ENVIRONMENTAL MICROBIOLOGY, 2003, 5 (09) :719-729
[6]   Updated version of the Burkholderia cepacia complex experimental strain panel [J].
Coenye, T ;
Vandamme, P ;
LiPuma, JJ ;
Govan, JRW ;
Mahenthiralingam, E .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (06) :2797-2798
[7]   Tackling both sides of the host-pathogen equation with Caenorhabditis elegans [J].
Ewbank, JJ .
MICROBES AND INFECTION, 2002, 4 (02) :247-256
[8]   Phenotypic methods for determining genomovar status of the Burkholderia cepacia complex [J].
Henry, DA ;
Mahenthiralingam, E ;
Vandamme, P ;
Coenye, T ;
Speert, DP .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (03) :1073-1078
[9]   Identification of Burkholderia cenocepacia genes required for bacterial survival in vivo [J].
Hunt, TA ;
Kooi, C ;
Sokol, PA ;
Valvano, MA .
INFECTION AND IMMUNITY, 2004, 72 (07) :4010-4022
[10]   Killing of Caenorhabditis elegans by Burkholderia cepacia is controlled by the cep quorum-sensing system [J].
Köthe, M ;
Antl, M ;
Huber, B ;
Stoecker, K ;
Ebrecht, D ;
Steinmetz, I ;
Eberl, L .
CELLULAR MICROBIOLOGY, 2003, 5 (05) :343-351
123