Determination of Cephalosporins by UHPLC-DAD Using Molecularly Imprinted Polymers

被引：20

作者：

Baeza-Fonte, Alen N. ^{[1
]}

Garces-Lobo, Idenia ^{[2
]}

Luaces-Alberto, Markel D. ^{[2
]}

Goncalves, Luis Moreira ^{[3
,4
]}

Sotomayor, Maria D. P. T. ^{[5
]}

Valdes-Gonzalez, Aristides C. ^{[1
]}

机构：

[1] Univ Havana, Inst Sci & Technol Mat, Universitary Lab Characterizat Struct Subst, Calle Zapata & G S-N, Havana 10400, Cuba

[2] Univ Havana, Fac Chem, Dept Analyt Chem, Calle Zapata & G S-N, Havana 10400, Cuba

[3] Univ Porto FCUP, Fac Ciencias, Dept Quim & Bioquim, REQUIMTE LAQV, Rua Campo Alegre S-N, P-4169007 Oporto, Portugal

[4] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Ave Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP, Brazil

[5] UNESP Univ Estadual Paulista, Inst Quim, Dept Quim Analit, Ave Prof Francisco Degni 55, BR-14800900 Araraquara, SP, Brazil

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2018年 / 56卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

SOLID-PHASE EXTRACTION; TANDEM MASS-SPECTROMETRY; WASTE-WATER; CAPILLARY-ELECTROPHORESIS; PHENOLIC-COMPOUNDS; ANTIBIOTICS; SAMPLES; MILK; CEPHALEXIN; RECOGNITION;

D O I：

10.1093/chromsci/bmx099

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Molecularly imprinted polymers (MIPs) were synthesized for the determination of cephalosporins (cephazolin, ceftazidime, cefotaxime, ceftriaxone, cefepime and cephalexin) by ultra-high performance liquid chromatography with diode-array detection (UHPLC-DAD). After optimization, MIPs were synthesized using cephazolin as the template, methacrylic acid as the functional monomer, triethylenglycol dimethacrylate as the crosslinker, acetonitrile/dimethylsulfoxide as porogens and benzoyl peroxide as the radical initiator. Not only this is a novel route of MIP synthesis for cephalosporins, but also this choice of analytes is unique. Chromatographic separation was performed in a C-8 column using a binary gradient with trifluoroacetic acid 0.1% in water and acetonitrile. Linearity was assessed up to 100 mu g mL(-1) and linear correlation coefficients (r) were all higher than 0.99, limits of detection were within the range of 3-12 ng mL(-1), and recoveries from 86 to 102% were obtained for concentrations between 0.05 and 1.0 mu g mL(-1).

引用

页码：187 / 193

页数：7