Metall(prote)omic studies by capillary electrophoresis using separation capillary as an in-line reactor

被引：15

作者：

Timerbaev, Andrei R. ^{[1
]}

Foteeva, Lidia S. ^{[1
]}

Pawlak, Katarzyna ^{[2
]}

Jarosz, Maciej ^{[2
]}

机构：

[1] Russian Acad Sci, Vernadsky Inst Geochem & Analyt Chem, Moscow 119991, Russia

[2] Warsaw Univ Technol, Chair Analyt Chem, PL-00664 Warsaw, Poland

来源：

METALLOMICS | 2011年 / 3卷 / 08期

关键词：

HETEROATOM-TAGGED PROTEOMICS; PLASMA-MASS SPECTROMETRY; EQUILIBRIUM MIXTURES; BINDING CONSTANTS; PROTEINS; KINETICS; CA2+; CE;

D O I：

10.1039/c1mt00007a

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This perspective article highlights the potential of capillary electrophoresis (CE) in in-line monitoring of biomolecular reactions related to in vivo transformations of metal species. In such scrutinizing, the capillary is regarded as a nanolitre-volume reactor in which electrical field-driven reactants are mixed to produce a response that enables in situ following-up and characterization of non-covalent molecular interactions. The concept of a CE reactor has been extended here to the investigation of processes that are responsible for the formation and decomposition of metal-bioligand species under simulated physiological conditions.

引用

页码：761 / 764

页数：4

共 21 条

[1] Nonequilibrium capillary electrophoresis of equilibrium mixtures - A single experiment reveals equilibrium and kinetic parameters of protein-DNA interactions [J].

Berezovski, M ;

Krylov, SN .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (46) :13674-13675

[2] Review on the theory of moving reaction boundary, electromigration reaction methods and applications in isoelectric focusing and sample pre-concentration [J].

Cao, Cheng-Xi ;

Fan, Liu-Yin ;

Zhang, Wei .

ANALYST, 2008, 133 (09) :1139-1157

[3] Different binding thermodynamics of Ni2+, CU2+, and Zn2+ to bacitracin A1 determined by capillary electrophoresis [J].

Castagnola, M ;

Rossetti, DV ;

Inzitari, R ;

Lupi, A ;

Zuppi, C ;

Cabras, T ;

Fadda, MB ;

Onnis, G ;

Petruzzelli, R ;

Giardina, B ;

Messana, I .

ELECTROPHORESIS, 2004, 25 (06) :846-852

[4] Binding of Ca2+, Mg2+, and heparin by human serum amyloid P component in affinity capillary electrophoresis [J].

Heegaard, Niels H. H. ;

He, Xinya ;

Blomberg, Lars G. .

ELECTROPHORESIS, 2006, 27 (13) :2609-2615

[5] A capillary electrophoretic reactor with an electroosmosis control method for measurement of dissociation kinetics of metal complexes [J].

Iki, N ;

Hoshino, H ;

Yotsuyanagi, T .

ANALYTICAL CHEMISTRY, 2000, 72 (20) :4812-4820

[6] Use of CE for the determination of binding constants [J].

Jiang, Chunxia ;

Armstrong, Daniel W. .

ELECTROPHORESIS, 2010, 31 (01) :17-27

[7] Non-equilibrium capillary electrophoresis of equilibrium mixtures - appreciation of kinetics in capillary electrophoresis [J].

Krylov, SN ;

Berezovski, M .

ANALYST, 2003, 128 (06) :571-575

[8] The Inject-Mix-React-Separate-and-Quantitate (IMReSQ) approach to studying reactions in capillaries [J].

Krylova, Svetlana M. ;

Okhonin, Victor ;

Evenhuis, Christopher J. ;

Krylov, Sergey N. .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2009, 28 (08) :987-1010

[9] Probing Nanoparticle-Protein Interaction by Capillary Electrophoresis [J].

Li, Ni ;

Zeng, Shang ;

He, Le ;

Zhong, Wenwan .

ANALYTICAL CHEMISTRY, 2010, 82 (17) :7460-7466

[10] Capillary electrophoresis with laser-induced fluorescence detection for chiral analysis and DNA binding studies of ruthenium(II) Tris-diimine complexes [J].

Martin, SE ;

Connatser, RM ;

Kane-Maguire, NAP ;

Wheeler, JF .

ANALYTICA CHIMICA ACTA, 2001, 445 (01) :21-27

← 1 2 3 →