A novel canine B-cell leukaemia cell line. Establishment, characterisation and sensitivity to chemotherapeutics

被引：22

作者：

Pawlak, A. ^{[1
]}

Ziolo, E. ^{[2
]}

Kutkowska, J. ^{[2
]}

Blazejczyk, A. ^{[3
]}

Wietrzyk, J. ^{[3
]}

Krupa, A. ^{[4
]}

Hildebrand, W. ^{[4
]}

Dziegiel, P. ^{[5
]}

Dzimira, S. ^{[6
]}

Obminska-Mrukowicz, B. ^{[1
]}

Strzadala, L. ^{[2
]}

Rapak, A. ^{[2
]}

机构：

[1] Wroclaw Univ Environm & Life Sci, Dept Biochem Pharmacol & Toxicol, Fac Vet Med, Wroclaw, Poland

[2] Ludwik Hirszfeld Inst Immunol & Expt Therapy, Lab Tumor Mol Immunobiol, Wroclaw, Poland

[3] Ludwik Hirszfeld Inst Immunol & Expt Therapy, Lab Expt Oncol, Wroclaw, Poland

[4] Wroclaw Univ Environm & Life Sci, Dept Internal Med & Clin Dis Horses Dogs & Cats, Wroclaw, Poland

[5] Wroclaw Med Univ, Dept Histol & Embryol, Wroclaw, Poland

[6] Wroclaw Univ Environm & Life Sci, Dept Pathol, Fac Vet Med, Wroclaw, Poland

来源：

VETERINARY AND COMPARATIVE ONCOLOGY | 2017年 / 15卷 / 04期

关键词：

B-CLL; canine cell line; canine leukaemia; xenograft; CHRONIC LYMPHOCYTIC-LEUKEMIA; NF-KAPPA-B; RETROVIRAL PARTICLES; LYMPHOMA; DOGS; MODEL; EXPRESSION; ANTIBODIES; APOPTOSIS; DIAGNOSIS;

D O I：

10.1111/vco.12257

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We established a new B-cell leukaemia cell line CLB70 from a dog with chronic lymphocytic leukaemia. This cell line is positive for CD20, CD45, CD79a, MHC class II, IgG, IgM; weakly positive for CD21; and negative for CD3, CD4, CD5, CD8, CD14, CD34, CD117. PCR for antigen receptor gene rearrangement (PARR) analysis revealed a biclonal immunoglobulin heavy chain (IgH) gene rearrangement and negative result for TCR. Western blot analysis of anti- and pro-apoptotic proteins showed increased expression of Bcl-2, Mcl-1, NF-kB, and Ras, and decreased expression of p53. CLB70 cells grow rapidly in vitro and are tumourigenic in nude mice. The CLB70 line is highly sensitive to doxorubicin, less sensitive to etoposide and imatinib, and resistant to piroxicam, celecoxib and dexamethasone. Our results indicate that CLB70 cells are derived from mature B-cells and they may be a useful tool for the development of new therapeutic strategies for both dogs and humans.

引用

页码：1218 / 1231

页数：14

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