The performance of Callosobruchus maculatus F. in artificial seeds containing proteins from Prosopis juliflora seeds

被引：2
|
作者：
Oliveira, AS
Lima, LM
Pereira, RA
Morais, AHA
Pereira, LS
Sales, MP
机构：
[1] Univ Fed Rio Grande do Norte, Ctr Biociencias, Dept Bioquim, Lab Quim, BR-59072970 Natal, RN, Brazil
[2] Univ Fed Rio Grande do Norte, Ctr Biociencias, Dept Bioquim, Funcao Prot, BR-59072970 Natal, RN, Brazil
[3] Univ Fed Ceara, Dept Bioquim & Biol Mol, Fortaleza, Ceara, Brazil
来源：
PROTEIN AND PEPTIDE LETTERS | 2001年 / 8卷 / 03期
关键词：
Prosopis juliflora DC; cysteine proteinase inhibitors; Callosobruchus maculatus; cowpea;
D O I：
10.2174/0929866013409490
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This work reportes the effects of isolated proteins from algaroba (Prosopis juliflora D.C.) seeds fractionated by ammonium sulfate at saturation 0-30%, 30-60% and 60-90%. They were tested, in vitro, against papain and midgut homogenate of C. maculatus larvae. Also, the effects in vivo these protein fractions on the development of C. maculatus larvae were tested. F 30/60 fraction was effective against papain (72% inhibition), midgut homogenate of larvae of C. maculatus (59,2% inhibition) and in vivo with WD50 of 1,7%.
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页码：225 / 229
页数：5
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