Gold Nanoparticle Based Electrochemical Immunosensor for Detection of T3 Hormone

被引：3

作者：

Saxena, Rahul ^{[1
]}

Fouad, H. ^{[2
,3
]}

Srivastava, Sudha ^{[1
]}

机构：

[1] Jaypee Inst Informat Technol, Nanobiotechnol Lab, Dept Biotechnol, A-10 Sect 62, Noida 201309, UP, India

[2] King Saud Univ, Community Coll, Appl Med Sci Dept, Riyadh 11433, Saudi Arabia

[3] Helwan Univ, Fac Engn, Biomed Engn Dept, Helwan 11792, Egypt

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2020年 / 20卷 / 10期

关键词：

Thyroid Disorder; Cyclic Voltammetry; Differential Pulse Voltammetry; Immunosensor; Gold Nanoparticles; THYROID-STIMULATING HORMONE; HYPOTHYROIDISM; GUIDELINES; ASSAY; SIZE;

D O I：

10.1166/jnn.2020.18561

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a nanoparticles based electrochemical immunosensor to detect and quantify triiodothyronine (T3) hormone. Immunosensor developed using gold nanoparticles and anti-T3 antibody, was employed for quantification of T3 antigen using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) technique. The electrochemical response of the developed immunosensor correlates well with the amount of antigen present in the sample. With increase in antigen concentration the immunocomplex formation on electrode surface increases and hence redox current decreases. The immunosensor shows a lower limit of detection of 1 pg/mL and dynamic range from 1 to 500 pg/mL. Sensitivity of the immunosensor was found to be 29.81 mu A/pg/mL/cm(2).

引用

页码：6057 / 6062

页数：6