Fluorescence imaging of membrane dynamics

被引：93
作者：
Groves, Jay T. [1 ,2 ,3 ]
Parthasarathy, Raghuveer [4 ]
Forstner, Martin B. [1 ]
机构：
[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Lawrence Berkeley Lab, Phys Biosci Div, Berkeley, CA 94720 USA
[3] Univ Calif Berkeley, Lawrence Berkeley Lab, Div Mat Sci, Berkeley, CA 94720 USA
[4] Univ Oregon, Dept Phys, Eugene, OR 97403 USA
来源：
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING | 2008年 / 10卷
关键词：
total internal reflection fluorescence microscopy; fluorescence interference contrast microscopy; fluorescence correlation spectroscopy; lipid membrane; cell membrane;
D O I：
10.1146/annurev.bioeng.10.061807.160431
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Imaging membrane dynamics is an important goal, motivated 1)), the abundance of biochemical and biophysical events that are orchestrated at or, by, cellular membranes. The short length scales, fast timescales, and environmental requirements of membrane phenomena present challenges to imaging experiments. Several technical advances offer means to overcome these challenges, and we describe here three powerful techniques applicable to membrane imaging: total internal reflection fluorescence (17IRF) microscopy, fluorescence interference contrast (FLIC) microscopy, and fluorescence correlation spectroscopy (FCS). For each, we discuss the physics underpinning the approach, its practical implementation, and recent examplcs highlighting its achievements in exploring the membrane environment.
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页码：311 / 338
页数：28
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