An intronic domain within the rat preprotachykinin-A gene containing a CCCT repetitive motif acts as an enhancer in differentiating embryonic stem cells

被引：9

作者：

Fiskerstrand, CE

Lovejoy, E

Gerrard, L

Quinn, JP

机构：

[1] Univ Edinburgh, Dept Vet Pathol, Edinburgh EH9 1QH, Midlothian, Scotland

[2] Univ Edinburgh, Dept Pathol, Edinburgh EH9 1QH, Midlothian, Scotland

来源：

NEUROSCIENCE LETTERS | 1999年 / 263卷 / 2-3期

基金：

英国生物技术与生命科学研究理事会;

关键词：

preprotachykinin; substance P; gene expression; transcription; embroynic stem cells; enhancer; intron; nociception;

D O I：

10.1016/S0304-3940(99)00127-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Previous attempts by several groups to clone fragments containing intron 2 of the rat preprotachykinin-A gene have generated deletions of various sizes. We have determined that these deletions occur within a specific region of the intron spanning a CCCT tandem repeat domain. We show that this intronic domain is able to support reporter gene expression in mouse embryonic stem (ES) cells that have been induced to differentiate but not in undifferentiated ES cells. No significant expression was observed in the HeLa clonal cell line. This demonstrates that this intron 2 domain is a highly restrictive enhancer and may be associated with differentiation. (C) 1999 Published by Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：141 / 144

页数：4

共 19 条

[1] EXPRESSION OF SUBSTANCE-P AND PREPROTACHYKININ MESSENGER-RNA BY PRIMARY SENSORY NEURONS IN CULTURE - REGULATION BY FACTORS PRESENT IN PERIPHERAL AND CENTRAL TARGET TISSUES
BARAKATWALTER, I
AFFOLTER, HU
DROZ, B
[J]. MOLECULAR BRAIN RESEARCH, 1991, 10 (02): : 107 - 114
[2] CARTER MS, 1990, J NEUROSCI, V10, P2203
[3] The molecular biology of preprotachykinin-A gene expression
Fiskerstrand, C
Quinn, JP
[J]. NEUROPEPTIDES, 1996, 30 (06) : 602 - 610
[4] SUBSTANCE-P GENE-EXPRESSION IS REGULATED BY INTERLEUKIN-1 IN CULTURED SYMPATHETIC-GANGLIA
HART, RP
SHADIACK, AM
JONAKAIT, GM
[J]. JOURNAL OF NEUROSCIENCE RESEARCH, 1991, 29 (03) : 282 - 291
[5] Tachykinins in the gut .1. Expression, release and motor function
Holzer, P
HolzerPetsche, U
[J]. PHARMACOLOGY & THERAPEUTICS, 1997, 73 (03) : 173 - 217
[6] MAGGIO JE, 1988, ANNU REV NEUROSCI, V11, P13, DOI 10.1146/annurev.neuro.11.1.13
[7] A NEW MOUSE EMBRYONIC STEM-CELL LINE WITH GOOD GERM LINE CONTRIBUTION AND GENE TARGETING FREQUENCY
MAGIN, TM
MCWHIR, J
MELTON, DW
[J]. NUCLEIC ACIDS RESEARCH, 1992, 20 (14) : 3795 - 3795
[8] REPRESSION OF PREPROTACHYKININ-A PROMOTER ACTIVITY IS MEDIATED BY A PROXIMAL PROMOTER ELEMENT
MENDELSON, SC
MORRISON, CF
MCALLISTER, J
PATERSON, JM
DOBSON, SP
MULDERRY, PK
QUINN, JP
[J]. NEUROSCIENCE, 1995, 65 (03) : 837 - 847
[9] CHARACTERIZATION OF POTENTIAL REGULATORY ELEMENTS WITHIN THE RAT PREPROTACHYKININ-A PROMOTER
MENDELSON, SC
QUINN, JP
[J]. NEUROSCIENCE LETTERS, 1995, 184 (02) : 125 - 128
[10] 5'-FLANKING SEQUENCES FROM THE RAT PREPROTACHYKININ GENE DIRECT HIGH-LEVEL EXPRESSION OF A REPORTER GENE IN ADULT-RAT SENSORY NEURONS TRANSFECTED IN CULTURE BY MICROINJECTION
MULDERRY, PK
CHAPMAN, KE
LYONS, V
HARMAR, AJ
[J]. MOLECULAR AND CELLULAR NEUROSCIENCE, 1993, 4 (02) : 164 - 172

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