Polyelectrolyte Capsules as Carriers for Growth Factor Inhibitor Delivery to Hepatocellular Carcinoma

被引：26

作者：

Baldassarre, Francesca ^{[2
]}

Vergaro, Viviana ^{[1
]}

Scarlino, Flavia ^{[1
]}

De Santis, Flavia ^{[1
]}

Lucarelli, Giovanna ^{[3
]}

della Torre, Antonio ^{[1
]}

Ciccarella, Giuseppe ^{[1
,3
]}

Rinaldi, Ross ^{[1
,3
]}

Giannelli, Gianluigi ^{[2
]}

Leporatti, Stefano ^{[1
]}

机构：

[1] CNR, NNL Ist Nanosci, I-73100 Lecce, Italy

[2] Univ Bari, Dept Internal Med Immunol & Infect Dis, Sect Internal Med, Sch Med, Bari, Italy

[3] Univ Salento, Dept Innovat Engn, I-73100 Lecce, Italy

来源：

MACROMOLECULAR BIOSCIENCE | 2012年 / 12卷 / 05期

关键词：

capsules; HCC; polyelectrolytes; TGF-beta inhibitor; IN-VIVO; CANCER-CELLS; CARBONATE MICROPARTICLES; MULTILAYER CAPSULES; COATED COLLOIDS; CELLULAR UPTAKE; FLOW-CYTOMETRY; DRUG-DELIVERY; MICROCAPSULES; VITRO;

D O I：

10.1002/mabi.201100457

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The efficient internalization of TGF-beta inhibitor-loaded polyelectrolyte capsules and particles is studied in two HCC cell lines. Two polyelectrolyte pairs (biocompatible but not degradable and biodegradable crosslinked with gluteraldehyde) are employed for coating. The capsules are characterized by SEM. LY is successfully loaded inside the core and embedded between polymer layers. MS is used to quantify the loading efficiency by comparing post-loading and core-loading methods, since both coated templates and hollow shells are used as carriers. CLSM confirms dissolution of the pre-formed multilayer upon enzymatic degradation as the method of release, and migration assays demonstrate a higher inhibition efficiency of TGF-beta in tailored biodegradable capsules compared to free LY administration.

引用

页码：656 / 665

页数：10

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