Characterization of a new methylated β-cyclodextrin with a low degree of substitution by electrospray ionization mass spectrometry and liquid chromatography/mass spectrometry

被引:15
作者
Jacquet, R [1 ]
Elfakir, C [1 ]
Lafosse, M [1 ]
机构
[1] Univ Orleans, CNRS, UMR 6005, Inst Chim Organ & Analyt,FR 2708,UFR Sci, F-45067 Orleans, France
关键词
D O I
10.1002/rcm.2175
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A new methylated beta-cyclodextrin (Me-beta-CD) with a low degree of substitution (DS) Was characterized by electrospray ionization mass spectrometry (ESI-MS) and liquid chromatography coupled with ESI-MS (LC/ESI-MS). For ESI-MS analyses, the composition of the infused sample solution was optimized in order to obtain only singly charged ammoniated CDs without fragmentation. The DS value (i.e. the number of methyl groups per glucopyranose unit) was found to be 0.7, which was in accordance with the values previously obtained by other methods. The LC/ESI-MS analysis, derived from a method using evaporative light scattering detection, allowed the study of the substitution isomers of each derivative and appears to be an easy and rapid tool for the accurate characterization of Me-beta-CD mixtures. Copyright (C) 2005 John Wiley & Sons, Ltd.
引用
收藏
页码:3097 / 3102
页数:6
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