Time-resolved X-ray scattering studies of proteins

被引：14

作者：

Cho, Hyun Sun ^{[1
]}

Schotte, Friedrich ^{[1
]}

Stadnytskyi, Valentyn ^{[1
]}

Anfinrud, Philip ^{[1
]}

机构：

[1] NIDDK, Chem Phys Lab, NIH, Bldg 2, Bethesda, MD 20892 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2021年 / 70卷

关键词：

Time-resolved; SAXS; WAXS; Protein structure dynamics; BIOLOGICAL MACROMOLECULES; STRUCTURAL DYNAMICS; HYDRATION LAYER; HEMOGLOBIN; SUBDOMAIN; KINETICS;

D O I：

10.1016/j.sbi.2021.05.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Time-resolved small- and wide-angle X-ray scattering studies of proteins in solution based on the pump-probe approach unveil structural information from intermediates over a broad range of length and time scales. In spite of the promise of this methodology, only a fraction of the wealth of information encoded in scattering data has been extracted in studies performed thus far. Here, we discuss the methodology, summarize results from recent time-resolved X-ray scattering studies, and examine the potential to extract additional information from these scattering curves.

引用

页码：99 / 107

页数：9

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