Development and validation of an ELISA method for detection of growth arrest specific 6 (GAS6) protein in human plasma

被引：31

作者：

Alciato, Federica ^{[1
]}

Sainaghi, Pier Paolo ^{[1
]}

Castello, Luigi ^{[1
]}

Bergamasco, Luca ^{[1
]}

Carnieletto, Stefania ^{[1
]}

Avanzi, Gian Carlo ^{[1
]}

机构：

[1] Univ Piemonte Orientale, Dept Clin & Expt Med, Novara, Italy

来源：

JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY | 2008年 / 29卷 / 02期

关键词：

Gas6; ELISA; development; assay; normal concentration; validation;

D O I：

10.1080/15321810801888480

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Gas6 protein is possibly involved in human diseases, but a validated plasma assay is lacking. So, we developed a sandwich enzyme-linked immunosorbent assay (ELISA) method using commercially available reagents. An appropriate plasma-based matrix was prepared to optimize the assay. The ELISA method showed inter- and intra-assay coefficients of variation lower than 15%. Recoveries all fell within 15% of expected values. Plasma Gas6 concentration in 61 healthy donors was 20.3 +/- 3.8 ng/mL. Our assay meets FDA requirements for precision and accuracy for the validation of bioanalytical methods and it is suitable for research or diagnostic purposes.

引用

页码：167 / 180

页数：14

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