The effect of age and length of gonadotropin stimulation on the in vitro embryo development of Holstein calf oocytes

被引：35

作者：

Currin, Luke ^{[1
]}

Michalovic, Laura ^{[1
]}

Bellefleur, Anne-Marie ^{[2
]}

Gutierrez, Karina ^{[1
]}

Glanzner, Werner ^{[1
]}

Schuermann, Yasmin ^{[1
]}

Bohrer, Rodrigo C. ^{[1
]}

Dicks, Naomi ^{[1
]}

da Rosa, Paulo R. ^{[1
]}

De Cesaro, Matheus P. ^{[1
]}

Lopez, Rosalba ^{[1
]}

Grand, Francois-Xavier ^{[2
]}

Vigneault, Christian ^{[2
]}

Blondin, Patrick ^{[2
]}

Gourdon, Jim ^{[3
]}

Baldassarre, Hernan ^{[1
]}

Bordignon, Vilceu ^{[1
]}

机构：

[1] McGill Univ, Dept Anim Sci, 21111,Lakeshore Rd,Room MS1-089, Ste Anne De Bellevue, PQ H9X 3V9, Canada

[2] Alliance Boviteq, St Hyacinthe, PQ, Canada

[3] McGill Univ, Comparat Med & Anim Resources Ctr, Montreal, PQ, Canada

来源：

THERIOGENOLOGY | 2017年 / 104卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

Dairy cattle; Prepubertal; Ovarian stimulation; Laparoscopic ovum pickup; Embryo development; OVUM PICK-UP; FOLLICULAR ASPIRATION; FERTILIZED OOCYTES; ADULT GOATS; COMPETENCE; CALVES; CAPACITY; JUVENILE; EMBRYOGENESIS; TECHNOLOGIES;

D O I：

10.1016/j.theriogenology.2017.08.011

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The use of oocytes recovered from prepubertal donors for in vitro embryo production has great potential for accelerating the rate of genetic gain in the dairy industry. However, these oocytes are known to be less developmentally competent than those from adult donors. In this study, we investigated the effect of age and gonadotropin stimulation in Holstein heifers subjected to oocyte collection every two weeks between 2 and 6 months of age. In order to assess the effect of gonadotropin stimulation, animals were subjected to one of three treatments, namely Short (ST; 36-42 h), Long (LT; >= 72 h) and No Treatment (NT) prior to laparoscopic ovum pick-up (LOPU). Our results show that the LT significantly improved the proportion of large follicles (>5 mm diameter) present in the ovary (LT 34.0% vs. ST 11.2% vs. NT 2.4%, P < 0.05), as well as the percentage of good-quality cumulus oocyte complexes (COCs) recovered (LT 953 +/- 18% vs. ST 85.4 +/- 22% vs. NT 82.2 +/- 14%, P < 0.05) and blastocyst rate (LT 36.7 +/- 26% vs. ST 18.3 +/- 15% vs. NT 16.7 +/- 9%, P < 0.05). Recovery rate was affected by treatment (LT 70.4 +/- 25 vs. ST 85.4 +/- 29 vs. NT 72.7 +/- 23, P < 0.05). To assess the impact of age, data was grouped into <100 days (A), 100-130 days (B) and >130 days (C) of age at LOPU. We found that as animals got older, although the average number of COCs per donor per LOPU declined (A: 17.5 +/- 11 vs. B: 14.7 +/- 7 vs. C: 11.9 +/- 8), the blastocyst rate increased (A: 12.8 +/- 20% vs. B: 17.1 +/- 21% vs. C: 21.8 +/- 25%, P < 0.05). We also evaluated the incidence of polyspermy and confirmed it is a critical limitation for IVF in calf oocytes. The incidence of polyspermy was unaffected by gonadotropin treatment, but significantly decreased with age. The capacity for full development to term of in vitro produced embryos from calf oocytes was tested by embryo transfer into 21 synchronized adult recipients, which resulted in 13 pregnancies (62%), full development to term and healthy calves born. Finally, the study allowed evaluating the safety of the procedure since, on average, each animal was subjected to 8 LOPU procedures over a period of 4 months. Our results showed that the procedure is safe (no incidents during laparoscopy), and was not harmful for the reproductive future of the animals, as those that were bred became pregnant after reaching sexual maturity. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：87 / 93

页数：7

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