Isothermal cross-priming amplification implementation study

被引：14

作者：

Bai, Z. ^{[1
]}

Xie, H. ^{[1
]}

You, Q. ^{[2
]}

Pickerill, S. ^{[2
]}

Zhang, Y. ^{[1
]}

Li, T. ^{[1
]}

Geng, J. ^{[1
]}

Hu, L. ^{[2
]}

Shan, H. ^{[3
]}

Di, B. ^{[1
]}

机构：

[1] Guangzhou Ctr Dis Control & Prevent, Guangzhou 510440, Guangdong, Peoples R China

[2] Ustar Biotechnol Hangzhou Co Ltd, Hangzhou, Zhejiang, Peoples R China

[3] ADICON Clin Lab Inc, Hangzhou, Zhejiang, Peoples R China

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2015年 / 60卷 / 03期

关键词：

Contamination-proofed; cross-priming amplification; isothermal amplification; rapid diagnostics; viral diarrhoea; ADENOVIRUS; DNA; INFECTIONS; ROTAVIRUS; CHILDREN;

D O I：

10.1111/lam.12342

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cross-priming amplification (CPA) was evaluated for the early detection of norovirus (NV), rotavirus A (RV-A), enteric adenovirus (EAdV) and astrovirus (AstV). The analytical sensitivity of the CPA assay was 10(3)copiesml(-1) for NV, RV-A and AstV detection and 10(4)copiesml(-1) for EAdV detection. For each of the four pathogens, the positive detection rate by CPA was similar to real-time PCR methods and higher than the rate observed in an ELISA. The detection coincidence rates of CPA and RT-PCR for NV, RV-A, EAdV and AstV were 98, 99, 99 and 100%, respectively. All CPA assays were negative in 89 healthy control samples. These results demonstrate the high analytical sensitivity and specificity of the CPA assay. CPA assays are relatively straightforward to perform, and such assays represent a potential detection method for locations in which resources are limited.

引用

页码：205 / 209

页数：5

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