Electrochemical Behavior of Calcein and the Interaction Between Calcein and DNA

被引：18

作者：

Zhang, Xuzhi ^{[1
]}

Li, Meng ^{[2
]}

Cui, Yi ^{[1
]}

Zhao, Jun ^{[1
]}

Cui, Zhengguo ^{[1
]}

Li, Qiufen ^{[1
]}

Qu, Keming ^{[1
]}

机构：

[1] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Shandong Prov Key Lab Fishery Resources & Ecol En, Qingdao 266071, Peoples R China

[2] Dalian Ocean Univ, Sch Life Sci & Technol, Dalian 116023, Peoples R China

来源：

ELECTROANALYSIS | 2012年 / 24卷 / 09期

基金：

中国国家自然科学基金;

关键词：

Calcein; Electrochemical behavior; DNA interaction; Voltammetry; Conductive carbon black paste electrode; MEDIATED ISOTHERMAL AMPLIFICATION; POTENTIAL SWEEP VOLTAMMETRY; CONDUCTIVE CARBON-BLACK; CALF THYMUS DNA; MALACHITE GREEN; LAMP; ELECTRODES; COMPLEXES; ASSAY; NEOMYCIN;

D O I：

10.1002/elan.201200192

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The electrochemical behavior of calcein (CA) has been investigated by using a conductive carbon black paste electrode (CCBPE) as working electrode. It exhibits a single well-defined redox peak in phosphate buffered saline in the range of pH 5.58.0, which attributes to the irreversible oxidation with 2 electrons and 2 protons participation. Under the optimized analytical conditions, the proposed linear sweep voltammetry (LSV) method allows the determination of CA in a linear concentration range of 0.649.60 mu M, with a limit of detection of 0.32 mu M. Further, the interaction between CA and DNA were studied by voltammetric and spectrometric methods. Both studies have shown that CA can bind to DNA by the intercalation binding mode. Under the present experimental condition, the binding constant beta of CA and dsDNA is 1.10X107. Meanwhile, in the loop-mediated isothermal amplification (LAMP) reaction mixture there is obvious interaction between CA and dsDNA, resulting in a nonignorable decrease of the indicating sensitivity.

引用

页码：1878 / 1886

页数：9

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