Inhibition of Yeast Growth During Long Term Exposure to Laser Light Around 1064 nm

被引：1

作者：

Aabo, Thomas ^{[1
]}

Perch-Nielsen, Ivan R. ^{[3
]}

Dam, Jeppe Seidelin ^{[2
]}

Palima, Darwin Z. ^{[2
]}

Siegumfeldt, Henrik ^{[1
]}

Gluckstad, Jesper ^{[2
]}

Arneborg, Nils ^{[1
]}

机构：

[1] Univ Copenhagen, Dept Food Sci, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark

[2] Tech Univ Denmark, Dept Photon Engn, DTU Foton, DK-4000 Roskilde, Denmark

[3] Tech Univ Denmark, Dept Micro & Nanotechnol, DTU Nanotech, DK-2800 Kongens Lyngby, Denmark

来源：

COMPLEX LIGHT AND OPTICAL FORCES III | 2009年 / 7227卷

关键词：

yeast; 1064; nm; inhibition; photodamage; growth; laser; Ytterbium; ESCHERICHIA-COLI; PARTICLES; DAMAGE;

D O I：

10.1117/12.810146

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

We have studied the effect of a 1070 nm continuous wave Ytterbium fiber laser on exponentially growing Saccharomyces cerevisiae yeast cells over a span of 4 hours. The cells were immobilized onto Concanavalin A covered microscope slides and the growth was measured using the area increase of the cells in 2D. Using a continuous dual beam plane wave with a uniform spatial intensity distribution, we found that a continuous radiant flux through a single cell as low as 0.5 mW in 1.5 hours significantly changed the growth and division rate of S. cerevisiae. With the dual beam setup used we were able to successfully manipulate single S. cerevisiae cells in 3 dimensions with a minimum flux thorough the cell of 3.5 mW. In the regime investigated from 0.7 mW to 2.6 mW we found no threshold for the photo damage, but rather a continuous response to the increased accumulated dose.

引用

页数：7

共 13 条

[1] Interactive optical trapping shows that confinement is a determinant of growth in a mixed yeast culture [J].

Arneborg, N ;

Siegumfeldt, H ;

Andersen, GH ;

Nissen, P ;

Daria, VR ;

Rodrigo, PJ ;

Glückstad, J .

FEMS MICROBIOLOGY LETTERS, 2005, 245 (01) :155-159

[2] Quantitative measurement of damage caused by 1064-nm wavelength optical trapping of Escherichia coli cells using on-chip single cell cultivation system [J].

Ayano, Satoru ;

Wakamoto, Yuichi ;

Yamashita, Shinobu ;

Yasuda, Kenji .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 350 (03) :678-684

[3] Three-dimensional imaging in three-dimensional optical multi-beam micromanipulation [J].

Dam, Jeppe Seidelin ;

Perch-Nielsen, Ivan R. ;

Palima, Darwin ;

Gluckstad, Jesper .

OPTICS EXPRESS, 2008, 16 (10) :7244-7250

[4] Microfluidics -: Sorting particles with light [J].

Glückstad, J .

NATURE MATERIALS, 2004, 3 (01) :9-10

[5] Bio-photonics Workstation [J].

Gluckstad, Jesper ;

Nielsen, Ivan P. ;

Dam, Jeppe S. ;

Palima, Darwin Z. .

COMPLEX LIGHT AND OPTICAL FORCES II, 2008, 6905 :A9050-A9050

[6] Wavelength dependence of cell cloning efficiency after optical trapping [J].

Liang, H ;

Vu, KT ;

Krishnan, P ;

Trang, TC ;

Shin, D ;

Kimel, S ;

Berns, MW .

BIOPHYSICAL JOURNAL, 1996, 70 (03) :1529-1533

[7] Optimal optical trap for bacterial viability [J].

Mirsaidov, Utkur ;

Timp, Winston ;

Timp, Kaethe ;

Mir, Mustafa ;

Matsudaira, Paul ;

Timp, Gregory .

PHYSICAL REVIEW E, 2008, 78 (02)

[8] 3-DIMENSIONAL OPTICAL TRAPPING AND LASER ABLATION OF A SINGLE POLYMER LATEX PARTICLE IN WATER [J].

MISAWA, H ;

KOSHIOKA, M ;

SASAKI, K ;

KITAMURA, N ;

MASUHARA, H .

JOURNAL OF APPLIED PHYSICS, 1991, 70 (07) :3829-3836

[9] Characterization of photodamage to Escherichia coli in optical traps [J].

Neuman, KC ;

Chadd, EH ;

Liou, GF ;

Bergman, K ;

Block, SM .

BIOPHYSICAL JOURNAL, 1999, 77 (05) :2856-2863

[10] Real-time interactive 3D manipulation of particles viewed in two orthogonal observation planes [J].

Perch-Nielsen, IR ;

Rodrigo, PJ ;

Glückstad, J .

OPTICS EXPRESS, 2005, 13 (08) :2852-2857

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