The effect of immobilization of heparin and bone morphogenic protein-2 (BMP-2) to titanium surfaces on inflammation and osteoblast function

被引：182
作者：
Kim, Sung Eun [2 ]
Song, Sang-Hun [1 ]
Yun, Young Pil [2 ]
Choi, Byung-Joon [1 ]
Kwon, Il Keun [3 ,4 ]
Bae, Min Soo [3 ,4 ]
Moon, Ho-Jin [3 ,4 ]
Kwon, Yong-Dae [1 ]
机构：
[1] Kyung Hee Univ, Sch Dent, Dept Oral & Maxillofacial Surg, Seoul 130701, South Korea
[2] Korea Univ, Coll Med, Guro Hosp, Dept Orthoped Surg & Rare Dis, Seoul 152703, South Korea
[3] Kyung Hee Univ, Sch Dent, Dept Maxillofacial Biomed Engn, Seoul 130701, South Korea
[4] Kyung Hee Univ, Sch Dent, Inst Oral Biol, Seoul 130701, South Korea
来源：
BIOMATERIALS | 2011年 / 32卷 / 02期
关键词：
Titanium; Heparin; Bone morphogenic protein 2 (BMP 2); Osteoblasts; Inflammation; Peri implantitis; MESENCHYMAL STEM-CELLS; LOW-MOLECULAR-WEIGHT; ACID-BASED HYDROGEL; ARTICULAR-CARTILAGE; SPINE FUSION; KAPPA-B; DEFECTS; DELIVERY; ADHESION; RHBMP-2;
D O I：
10.1016/j.biomaterials.2010.09.008
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
The aim of this study was to investigate biologic function of bone morphorgenic protein-2 (rhBMP-2) immobilized on the heparin-grafted Ti surface Ti surfaces were first modified by 3-amino-propyltriethoxysilane (ATPES) followed by grafting of heparin BMP-2 was then immobilized on the heparin-grafted Ti surfaces Pristine Ti and functionalized Ti surfaces were characterized by X-ray photoelectron spectroscopy (XPS) measurement of water contact angles and protein adsorption The biological activity of MG-63 cells on pristine and functionalized Ti surfaces was investigated by cell proliferation assays measurement of alkaline phosphate (ALP) activity and determination of calcium deposition Anti-inflammatory effects were assessed by RT-PCR to measure the transcript levels of IL-6 and TNF-alpha XPS revealed that heparin and BMP-2 were successfully grafted and immobilized on the Ti surfaces respectively In addition Ti surfaces with BMP-2 immobilized were more hydrophilic than pristine Ti Furthermore BMP-2 immobilized Ti promoted significantly higher ALP activity and calcium deposition by MG-63 cells than pristine Ti The inflammatory response was also decreased when cells were grown on heparin-grafted BMP-2-immobilized Ti surfaces The results of this study suggest that by grafting heparin and immobilizing BMP-2 on Ti surfaces inflammation can be inhibited and osteoblast function promoted (C) 2010 Elsevier Ltd All rights reserved
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页码：366 / 373
页数：8
相关论文
共 39 条
  • [1] Increased osteoblast adhesion on nanograined Ti modified with KRSR
    Balasundaram, Ganesan
    Webster, Thomas J.
    [J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2007, 80A (03) : 602 - 611
  • [2] Fluid flow increases mineralized matrix deposition in 3D perfusion culture of marrow stromal osteloblasts in a dose-dependent manner
    Bancroft, GN
    Sikavitsast, VI
    van den Dolder, J
    Sheffield, TL
    Ambrose, CG
    Jansen, JA
    Mikos, AG
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) : 12600 - 12605
  • [3] Bone morphogenetic protein-2 increases the rate of callus formation after fracture of the rabbit tibia
    Bax, BE
    Wozney, JM
    Ashhurst, DE
    [J]. CALCIFIED TISSUE INTERNATIONAL, 1999, 65 (01) : 83 - 89
  • [4] A systematic review of the incidence of biological and technical complications in implant dentistry reported in prospective longitudinal studies of at least 5 years
    Berglundh, T
    Persson, L
    Klinge, B
    [J]. JOURNAL OF CLINICAL PERIODONTOLOGY, 2002, 29 : 197 - 212
  • [5] Berglundh T., 2002, J Clin Periodontol, V29, P197212
  • [6] Heparin immobilized porous PLGA microspheres for angiogenic growth factor delivery
    Chung, Hyun Jung
    Kim, Hong Kee
    Yoon, Jun Jin
    Park, Tae Gwan
    [J]. PHARMACEUTICAL RESEARCH, 2006, 23 (08) : 1835 - 1841
  • [7] EFFECT OF RECOMBINANT HUMAN OSTEOGENIC PROTEIN-1 ON HEALING OF SEGMENTAL DEFECTS IN NONHUMAN-PRIMATES
    COOK, SD
    WOLFE, MW
    SALKELD, SL
    RUEGER, DC
    [J]. JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1995, 77A (05) : 734 - 750
  • [8] Recombinant human bone morphogenetic protein-2 for treatment of open tibial fractures -: A prospective, controlled, randomized study of four hundred and fifty patients
    Govender, S
    Csimma, C
    Genant, HK
    Valentin-Opran, A
    Amit, Y
    Arbel, R
    Aro, H
    Atar, D
    Bishay, M
    Börner, MG
    Chiron, P
    Choong, P
    Cinats, J
    Courtenay, B
    Feibel, R
    Geulette, B
    Gravel, C
    Haas, N
    Raschke, M
    Hammacher, E
    van der Velde, D
    Hardy, P
    Holt, M
    Josten, C
    Ketterl, RL
    Lindeque, B
    Lob, G
    Mathevon, H
    Mccoy, G
    Marsh, D
    Miller, R
    Munting, E
    Oevre, S
    Nordsletten, L
    Patel, A
    Pohl, A
    Rennie, W
    Reynders, P
    Rommens, PM
    Rondia, J
    Rossouw, WC
    Daneel, PJ
    Ruff, S
    Rüter, A
    Santavirta, S
    Schildhauer, TA
    Gekle, C
    Schnettler, R
    Segal, D
    Seiler, H
    [J]. JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 2002, 84A (12) : 2123 - 2134
  • [9] Heparin-functionalized chitosan-alginate scaffolds for controlled release of growth factor
    Ho, Yi-Cheng
    Mi, Fwu-Long
    Sung, Hsing-Wen
    Kuo, Pi-Li
    [J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2009, 376 (1-2) : 69 - 75
  • [10] Low-molecular weight and unfractionated heparins induce a downregulation of inflammation:: decreased levels of proinflammatory cytokines and nuclear factor-κB in LPS-stimulated human monocytes
    Hochart, H
    Jenkins, PV
    Smith, OP
    White, B
    [J]. BRITISH JOURNAL OF HAEMATOLOGY, 2006, 133 (01) : 62 - 67
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