Astrocytes detect and upregulate transmission at inhibitory synapses of somatostatin interneurons onto pyramidal cells

被引:81
作者
Matos, Marco [1 ,2 ]
Bosson, Anthony [1 ,2 ]
Riebe, Ilse [1 ,2 ]
Reynell, Clare [1 ,2 ]
Vallee, Joanne [1 ,2 ]
Laplante, Isabel [1 ,2 ]
Panatier, Aude [3 ,4 ]
Robitaille, Richard [1 ,2 ]
lacaille, Jean-ClauDe [1 ,2 ]
机构
[1] Univ Montreal, Fac Med, Dept Neurosci, POB 6128,Stn Ctr Ville, Montreal, PQ H3C 3J7, Canada
[2] Univ Montreal, Grp Rech Syst Nerveux Cent, POB 6128,Stn Ctr Ville, Montreal, PQ H3C 3J7, Canada
[3] INSERM, U1215, Neuroctr Magendie, F-33077 Bordeaux, France
[4] Univ Bordeaux, F-33077 Bordeaux, France
基金
加拿大自然科学与工程研究理事会;
关键词
HIPPOCAMPUS IN-VITRO; RAT HIPPOCAMPUS; SYNAPTIC-TRANSMISSION; HETEROSYNAPTIC DEPRESSION; DENDRITIC INHIBITION; GABAERGIC SYNAPSES; GABA TRANSPORTERS; CEREBRAL-CORTEX; CA1; REGION; SLEEP LOSS;
D O I
10.1038/s41467-018-06731-y
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Astrocytes are important regulators of excitatory synaptic networks. However, astrocytes regulation of inhibitory synaptic systems remains ill defined. This is particularly relevant since GABAergic interneurons regulate the activity of excitatory cells and shape network function. To address this issue, we combined optogenetics and pharmacological approaches, twophoton confocal imaging and whole-cell recordings to specifically activate hippocampal somatostatin or paravalbumin-expressing interneurons (SOM-INs or PV-INs), while monitoring inhibitory synaptic currents in pyramidal cells and Ca2+ responses in astrocytes. We found that astrocytes detect SOM-IN synaptic activity via GABABR and GAT-3-dependent Ca2+ signaling mechanisms, the latter triggering the release of ATP. In turn, ATP is converted into adenosine, activating A1Rs and upregulating SOM-IN synaptic inhibition of pyramidal cells, but not PV-IN inhibition. Our findings uncover functional interactions between a specific subpopulation of interneurons, astrocytes and pyramidal cells, involved in positive feedback autoregulation of dendritic inhibition of pyramidal cells.
引用
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页数:15
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