Protein-protein interactions in the subunits of ribonuclease p in the hyperthermophilic archaeon Pyrococcus horikoshii OT3

被引：27

作者：

Kifusa, M ^{[1
]}

Fukuhara, H ^{[1
]}

Hayashi, T ^{[1
]}

Kimura, M ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch, Fac Agr, Dept Biosci & Biotechnol,Lab Biochem, Fukuoka 8128581, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2005年 / 69卷 / 06期

关键词：

archaea; Pyrococcus horikoshii; protein-protein interaction; ribonuclease P; two-hybrid system;

D O I：

10.1271/bbb.69.1209

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ribonuclease P (RNase P) is a ribonucleoprotein complex involved in the processing of the 5' leader sequence of precursor tRNA (pre-tRNA). RNase P in the hyperthermophilic archaeon Pyrococcus horikoshii OT3 consists of RNA and five protein subunits (Ph1481p, Ph1496p, Ph1601p, Ph1771p, and Ph1877p). In vivo interactions among five protein subunits of RNase P in A horikoshii OT3 were examined using a yeast two-hybrid system. The analysis indicates that proteins Ph1481p and Ph1601p interact strongly with Ph1877p and Ph1771p respectively, whereas Ph1481p interacts moderately with Ph1601p. In contrast, no interaction was detected between Ph1496p and the other four proteins. Co-immunoprecipitation analysis confirmed the. interactions obtained by yeast two-hybrid assay.

引用

页码：1209 / 1212

页数：4

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