Interpretation of Real-Time PCR Results for Hepatitis C Virus RNA When Viral Load Is Below Quantification Limits

被引：8

作者：

Laperche, Syria ^{[1
]}

Bouchardeau, Francoise ^{[1
]}

Andre-Garnier, Elisabeth ^{[2
]}

Thibault, Vincent ^{[3
]}

Roque-Afonso, Anne-Marie ^{[4
]}

Trimoulet, Pascale ^{[5
]}

Colimon, Ronald ^{[6
]}

Duverlie, Gilles ^{[7
]}

Leguillou-Guillemette, Helene ^{[8
]}

Lunel, Francoise ^{[8
]}

Bouvier-Alias, Magali ^{[9
]}

Pawlotsky, Jean-Michel ^{[9
]}

Henquell, Cecile ^{[10
]}

Schvoerer, Evelyne ^{[11
]}

Stoll-Keller, Francoise ^{[11
]}

Chaix, Marie-Laure ^{[12
]}

Branger, Michel ^{[13
]}

Gaudy-Graffin, Catherine ^{[14
]}

Rosenberg, Arielle R. ^{[15
,16
]}

Pozzetto, Bruno ^{[17
]}

Vallet, Sophie ^{[18
]}

Baazia, Yazid ^{[19
]}

Izopet, Jacques ^{[20
]}

Lefrere, Jean-Jacques ^{[1
,21
]}

机构：

[1] Inst Natl Transfus Sanguine, Dept Agents Transmissibles Sang, Ctr Natl Reference Hepatites B&C Transfus, 6 Rue Alexandre Cabanel, F-75015 Paris, France

[2] Hop Hotel Dieu, Inst Biol, Nantes, France

[3] Ctr Hosp Univ Pitie Salpetriere, Virol Lab, Paris, France

[4] Ctr Hosp Paul Brousse, Virol Lab, Villejuif, France

[5] Ctr Hosp Univ, Virol Lab, Bordeaux, France

[6] Ctr Hosp Univ, Virol Lab, Rennes, France

[7] Ctr Hosp Univ, Virol Lab, Amiens, France

[8] Ctr Hosp Univ Angers, Lab Virol Bacteriol, Angers, France

[9] Ctr Hosp Univ Henri Mondor, Lab Virol Bacteriol, Creteil, France

[10] Ctr Hosp Univ, Virol Lab, Clermont Ferrand, France

[11] Ctr Hosp Univ, Virol Lab, Strasbourg, France

[12] Ctr Hosp Univ Necker, Virol Lab, Paris, France

[13] Ctr Hosp Univ Bichat, Virol Lab, Paris, France

[14] Ctr Hosp Univ Tours, INSERM, ERI19, Tours, France

[15] Univ Paris 05, Fac Med, Paris, France

[16] Grp Hosp Cochin, AP HP, Paris, France

[17] Ctr Hosp Univ, Lab Virol Bacteriol, St Etienne, France

[18] Ctr Hosp Univ, Microbiol Lab, Brest, France

[19] Ctr Hosp Univ Avicenne, Associe CNR Hepatites B&C, Lab Virol Bacteriol, Bobigny, France

[20] Ctr Hosp Univ, Virol Lab, Toulouse, France

[21] Ctr Hosp Univ, Hematol Lab, Amiens, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 03期

关键词：

ANALYTE-SPECIFIC REAGENT; ASSAY;

D O I：

10.1128/JCM.01511-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hepatitis C virus RNA quantification results obtained in 18 laboratories using real-time PCR methods with 10 negative samples and 22 sample dilutions (viral loads of 0.5 to 500 IU/ml) showed a score of correct results of up to 93.5%. However, 55.6% of the laboratories did not follow the recommendations for the interpretation of their results, leading to ambiguous conclusions.

引用

页码：1113 / 1115

页数：3

共 8 条

[1] Performance characteristics of a quantitative TaqMan hepatitis C virus RNA analyte-specific reagent [J].