Gamma-cross-linked nonfibrillar collagen gel as a scaffold for osteogenic differentiation of mesenchymal stem cells

被引：26

作者：

Takitoh, Takako ^{[1
]}

Bessho, Masahiko ^{[2
]}

Hirose, Motohiro ^{[3
]}

Ohgushi, Hajime ^{[4
]}

Mori, Hideki ^{[1
]}

Hara, Masayuki ^{[1
]}

机构：

[1] Osaka Prefecture Univ, Grad Sch Sci, Dept Biol Sci, Naka Ku, Sakai, Osaka 5998570, Japan

[2] Akita Univ, Int Ctr Res & Educ Mineral & Energy Resources, Akita 0108502, Japan

[3] Natl Inst Adv Ind Sci & Technol, Human Life Technol Res Inst, Tsukuba, Ibaraki 3058566, Japan

[4] Natl Inst Adv Ind Sci & Technol, Hlth Res Inst, Amagasaki, Hyogo 6610974, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2015年 / 119卷 / 02期

关键词：

Collagen fibril; Bone formation; Osteoblast; Bone marrow stromal cell; Mesenchymal stem cell; IN-VITRO; HUMAN OSTEOSARCOMA; BONE; MINERALIZATION; ALPHA-2-BETA-1; VISUALIZATION; IRRADIATION; PROTEINS; FIBRILS; CULTURE;

D O I：

10.1016/j.jbiosc.2014.07.008

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We fabricated a transparent nonfibrillar collagen gel using gamma irradiation (5 kGy) and cultured rat mesenchymal stem cells (MSCs) on both the gamma-irradiated collagen gel and on unirradiated fibrillar collagen gel. Cells attached well and proliferated with high viability on the surface of both gels. The cells cultured on the gamma-irradiated nonfibrillar gel had a unique elongated shape and adhered to each other in culture. After 21 days of culture in dexamethasone-containing culture medium, the contents of bone-specific osteocalcin and calcium on the gamma-irradiated nonfibrillar gel were 1.4 and 1.9 times higher than those on fibrillar collagen gel, respectively. These data show that osteogenic differentiation of MSCs was promoted more efficiently on the gamma-cross-linked nonfibrillar gel than on the fibrillar gel and demonstrate the potential of the gamma-irradiated collagen gel for use in bone tissue engineering. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：217 / 225

页数：9

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