A role for microRNAs in maintenance of mouse mammary epithelial progenitor cells

被引：179
作者：
Ibarra, Ingrid [1 ,2 ]
Erlich, Yaniv [1 ]
Muthuswamy, Senthil K. [1 ]
Sachidanandam, Ravi [1 ]
Hannon, Gregory J. [1 ]
机构：
[1] Cold Spring Harbor Lab, Howard Hughes Med Inst, Watson Sch Biol Sci, Cold Spring Harbor, NY 11724 USA
[2] SUNY Stony Brook, Genet Program, Stony Brook, NY 11794 USA
来源：
GENES & DEVELOPMENT | 2007年 / 21卷 / 24期
关键词：
RNAi; microRNA; stem cell; ALDEHYDE DEHYDROGENASE-ACTIVITY; HEMATOPOIETIC STEM-CELLS; ACUTE MYELOID-LEUKEMIA; IDENTIFICATION; LINE; RNA; EXPRESSION; MATURATION; RESISTANCE; DROSOPHILA;
D O I：
10.1101/gad.1616307
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
microRNA (miRNA) expression profiles are often characteristic of specific cell types. The mouse mammary epithelial cell line, Comma-D beta, contains a population of self-renewing progenitor cells that can reconstitute the mammary gland. We purified this population and determined its miRNA signature. Several microRNAs, including miR-205 and miR-22, are highly expressed in mammary progenitor cells, while others, including let-7 and miR-93, are depleted. Let-7 sensors can be used to prospectively enrich self-renewing populations, and enforced let-7 expression induces loss of self-renewing cells from mixed cultures.
引用
收藏
页码：3238 / 3243
页数：6
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