Virucidal activity of human α- and β-defensins against hepatitis C virus genotype 4

Hepatitis C virus (HCV) is the major etiological agent of human non-A and non-B hepatitis affecting about 180 million people worldwide. The goal of the current study was to find effective anti-HCV proteins. As a result, defensins were selected as promising candidates due to their well-known anti-viral potential and small size. We conducted in vitro evaluation of two kinds of defensins (human alpha- and beta-defensins and synthetic linear avian alpha-defensins) using tissue culture combined with reverse transcription nested PCR (RT-nested-PCR) and real-time PCR. Human alpha- and beta-defensins showed strong anti-HCV activity in experiments on cellular protection, neutralization, and treatment at all concentrations used (10, 20 and 50 mu g). The synthetic linear defensins could reach similar anti-HCV potential only at a noticeably higher concentration (250 mu g) and do not show noticeable activity at 10 and 20 mu g. This study suggests that defensins are potent anti-HCV agents.