Iron-based ferritin nanocore as a contrast agent

被引：43

作者：

Sana, Barindra ^{[1
]}

Johnson, Eric ^{[3
]}

Sheah, Kenneth ^{[2
]}

Poh, Chueh Loo ^{[1
]}

Lim, Sierin ^{[1
]}

机构：

[1] Nanyang Technol Univ, Sch Chem & Biomed Engn, Div Bioengn, Singapore 637457, Singapore

[2] Radlink Diagnost Imaging, Paragon Med, Singapore 238859, Singapore

[3] CALTECH, Howard Hughes Med Inst, Div Chem & Chem Engn, Pasadena, CA 91125 USA

来源：

BIOINTERPHASES | 2010年 / 5卷 / 03期

关键词：

SERUM FERRITIN; LIVER; MRI; RELAXIVITY; NANOPARTICLES; APOFERRITIN; THALASSEMIA; MECHANISM; STORAGE;

D O I：

10.1116/1.3483216

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Self-assembling protein cages have been exploited as templates for nanoparticle synthesis. The ferritin molecule, a protein cage present in most living systems, stores excess soluble ferrous iron in the form of an insoluble ferric complex within its cavity. Magnetic nanocores formed by loading excess iron within an engineered ferritin from Archaeoglobus fulgidus (AfFtn-AA) were studied as a potential magnetic resonance (MR) imaging contrast agent. The self-assembly characteristics of the AfFtn-AA were investigated using dynamic light scattering technique and size exclusion chromatography. Homogeneous size distribution of the assembled nanoparticles was observed using transmission electron microscopy. The magnetic properties of iron-loaded AfFtn-AA were studied using vibrating sample magnetometry. Images obtained from a 3.0 T whole-body MRI scanner showed significant brightening of T-1 images and signal loss of T-2 images with increased concentrations of iron-loaded AfFtn-AA. The analysis of the MR image intensities showed extremely high R-2 values (5300 mM(-1) s(-1)) for the iron-loaded AfFtn-AA confirming its potential as a T-2 contrast agent. (C) 2010 American Vacuum Society. [DOI: 10.1116/1.3483216]

引用

页码：FA48 / FA52

页数：5

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