Identification of basic fibroblast growth factor-like immunoreactivity in Panax ginseng extract: Investigation of its molecular properties

被引：7
|
作者：
Takei, Y [1 ]
Yamamoto, T [1 ]
Higashira, H [1 ]
Hayashi, K [1 ]
机构：
[1] GIFU PHARMACEUT UNIV, DEPT BIOL MOLEC, GIFU 502, JAPAN
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 04期
关键词：
basic fibroblast growth factor (bFGF); Panax ginseng; enzyme immunoassay (EIA); purification; mitogenic activity;
D O I：
10.1271/bbb.60.584
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Basic fibroblast growth factor (bFGF)-like immunoreactivity was detected in extracts of Panax ginseng root by using a sensitive two-site enzyme immunoassay specific for human bFGF (hbFGF). In an investigation of the molecular properties of this bFGF-like molecule (bFGF-LI), the bFGF-LI and hbFGF were found to be equivalent with respect to antigenicity, molecular weight, isoelectric point, affinity for binding to heparin, and mitogenic activity toward BALB/c3T3 fibroblasts. The identification of this bFGF-LI molecule in Panax ginseng root helps to explain various activities of the traditional Chinese medicine ginseng.
引用
收藏
页码：584 / 588
页数：5
相关论文
共 50 条
  • [41] GLIAL-CELL LOCALIZATION OF ACIDIC FIBROBLAST GROWTH FACTOR-LIKE IMMUNOREACTIVITY IN THE OPTIC-NERVE OF YOUNG-ADULT AND AGED MAMMALS
    FAUCHEUX, BA
    COHEN, SY
    DELAERE, P
    TOURBAH, A
    DUPUIS, C
    HARTMANN, MP
    JEANNY, JC
    HAUW, JJ
    COURTOIS, Y
    GERONTOLOGY, 1992, 38 (06) : 308 - &
  • [42] Identification of a novel domain of fibroblast growth factor 2 controlling its angiogenic properties
    Facchiano, A
    Russo, K
    Facchiano, AM
    De Marchis, F
    Facchiano, F
    Ribatti, D
    Aguzzi, MS
    Capogrossi, MC
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (10) : 8751 - 8760
  • [43] Tumor necrosis factor-like weak inducer of apoptosis and its receptor fibroblast growth factor-inducible 14 are expressed in urticarial vasculitis
    Li, Mengmeng
    Chen, Tao
    Guo, Zaipei
    Li, Jingyi
    Cao, Na
    JOURNAL OF DERMATOLOGY, 2013, 40 (11): : 891 - 895
  • [44] T-LYMPHOCYTES THAT INFILTRATE TUMORS AND ATHEROSCLEROTIC PLAQUES PRODUCE HEPARIN-BINDING EPIDERMAL GROWTH FACTOR-LIKE GROWTH-FACTOR AND BASIC FIBROBLAST GROWTH-FACTOR - A POTENTIAL PATHOLOGICAL ROLE
    PEOPLES, GE
    BLOTNICK, S
    TAKAHASHI, K
    FREEMAN, MR
    KLAGSBRUN, M
    EBERLEIN, TJ
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (14) : 6547 - 6551
  • [45] Platelet-derived growth factor inhibits basic fibroblast growth factor angiogenic properties in vitro and in vivo through its α receptor
    De Marchis, F
    Ribatti, D
    Giampietri, C
    Lentini, A
    Faraone, D
    Scoccianti, M
    Capogrossi, MC
    Facchiano, A
    BLOOD, 2002, 99 (06) : 2045 - 2053
  • [46] AORTIC ENDOTHELIAL-CELLS SYNTHESIZE BASIC FIBROBLAST GROWTH-FACTOR WHICH REMAINS CELL ASSOCIATED AND PLATELET-DERIVED GROWTH FACTOR-LIKE PROTEIN WHICH IS SECRETED
    VLODAVSKY, I
    FRIDMAN, R
    SULLIVAN, R
    SASSE, J
    KLAGSBRUN, M
    JOURNAL OF CELLULAR PHYSIOLOGY, 1987, 131 (03) : 402 - 408
  • [47] Identification of Basic Fibroblast Growth Factor responsive elements in the rat Insulin-like Growth Factor Binding Protein-5 gene.
    Yeh, LCC
    Yeh, V
    Lee, JC
    FASEB JOURNAL, 1999, 13 (07): : A1504 - A1504
  • [48] BOVINE BASIC FIBROBLAST GROWTH-FACTOR - IDENTIFICATION AND BINDING OF ITS RECEPTOR ON PC12 CELLS
    SHARMA, A
    DAHIYA, R
    BIOMEDICA BIOCHIMICA ACTA, 1988, 47 (12) : 975 - 983
  • [49] Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution
    Dallard, Bibiana E.
    Pujato, Silvina A.
    Baravalle, Celina
    Pereyra, Elizabet A. L.
    Rey, Florencia
    Renna, Maria S.
    Calvinho, Luis F.
    RESEARCH IN VETERINARY SCIENCE, 2013, 94 (03) : 462 - 470
  • [50] Identification of a new polymorphism in the basic fibroblast growth factor gene: investigation of the genotype frequencies in patients with proliferative vascular disease
    Handschug, K
    Schulz, S
    Kottke, R
    Boehm, U
    Schnuerer, C
    Koehler, S
    Hagemann, M
    Teichmann, W
    Glaeser, C
    AMERICAN JOURNAL OF HUMAN GENETICS, 1997, 61 (04) : A174 - A174
12345