Identification of basic fibroblast growth factor-like immunoreactivity in Panax ginseng extract: Investigation of its molecular properties

被引：7

作者：

Takei, Y ^{[1
]}

Yamamoto, T ^{[1
]}

Higashira, H ^{[1
]}

Hayashi, K ^{[1
]}

机构：

[1] GIFU PHARMACEUT UNIV, DEPT BIOL MOLEC, GIFU 502, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 04期

关键词：

basic fibroblast growth factor (bFGF); Panax ginseng; enzyme immunoassay (EIA); purification; mitogenic activity;

D O I：

10.1271/bbb.60.584

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Basic fibroblast growth factor (bFGF)-like immunoreactivity was detected in extracts of Panax ginseng root by using a sensitive two-site enzyme immunoassay specific for human bFGF (hbFGF). In an investigation of the molecular properties of this bFGF-like molecule (bFGF-LI), the bFGF-LI and hbFGF were found to be equivalent with respect to antigenicity, molecular weight, isoelectric point, affinity for binding to heparin, and mitogenic activity toward BALB/c3T3 fibroblasts. The identification of this bFGF-LI molecule in Panax ginseng root helps to explain various activities of the traditional Chinese medicine ginseng.

引用

页码：584 / 588

页数：5

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